An-Najah Staff

The aim of this study was to carry an ethnopharmacological survey on medicinal herbs and the methodology used in extraction of active compounds. A survey study was carried out; it included herbalists, herbal shops and people who are involved in traditional Arabic medicine. For each disease included, informants were asked to list plants used, the used part from which the products were prepared as well as the method of preparation. A total of 109 plants were identified. Within the plants used, leaves (47.3%), fruits (18.5%) and seeds (18.0%) were the plant parts most widely used. Methods of preparation were mainly decoction (boiling) by 51%, and then infusion (drenching) by 17%. Some plants were prepared as creams, powders, syrups, added to food or cooked. Many plant species are still used by herbalists in our country for treating various human diseases and ailments. Most plants are prepared by boiling, which may cause degradation of active ingredients. Preparations should take into consideration the stability of the active ingredients.

The aim of this study was to carry an ethnopharmacological survey on medicinal herbs and the methodology used in extraction of active compounds. A survey study was carried out; it included herbalists, herbal shops and people who are involved in traditional Arabic medicine. For each disease included, informants were asked to list plants used, the used part from which the products were prepared as well as the method of preparation. A total of 109 plants were identified. Within the plants used, leaves (47.3%), fruits (18.5%) and seeds (18.0 %) were the plant parts most widely used. Methods of preparation were mainly decoction (boiling) by 51%, and then infusion (drenching) by 17%. Some plants were prepared as creams, powders, syrups, added to food or cooked. Many plant species are still used by herbalists in our country for treating various human diseases and ailments. Most plants are prepared by boiling, which may cause degradation of active ingredients. Preparations should take into consideration the stability of the active ingredients.

The aim of this study was to carry an ethnopharmacological survey on medicinal herbs and the methodology used in extraction of active compounds. A survey study was carried out; it included herbalists, herbal shops and people who are involved in traditional Arabic medicine. For each disease included, informants were asked to list plants used, the used part from which the products were prepared as well as the method of preparation. A total of 109 plants were identified. Within the plants used, leaves (47.3%), fruits (18.5%) and seeds (18.0%) were the plant parts most widely used. Methods of preparation were mainly decoction (boiling) by 51%, and then infusion (drenching) by 17%. Some plants were prepared as creams, powders, syrups, added to food or cooked. Many plant species are still used by herbalists in our country for treating various human diseases and ailments. Most plants are prepared by boiling, which may cause degradation of active ingredients. Preparations should take into consideration the stability of the active ingredients.

Purpose: The aim of this study is to develop bromhexine  hydrochloride 1 %w/v oral solution for veterinary use and to evaluate its  stability. Methods: Solutions of Bromhexine hydrochloride (1%w/v) were  prepared by dissolving bromhexine hydrochloride in benzyl alcohol at 50 °C  then alcohol 96 % v/v; Tween 80 and purified water were added. The obtained  solution was filled in amber glass bottles, and the solution was stored at 25  °C/60 % relative humidity (RH) and at 40 °C /75% RH. The strengths of  bromhexine hydrochloride were determined by High performance liquid  chromatographic assay at 0, 2, 4, 6, 8, 10, 12, 16, 20 and 24 months. The  concentrations of the drug were directly related to the peak area. pH, odor,  color and crystal formation was also monitored. Results: The degradation of  bromhexine hydrochloride 1% w/v oral solution was faster at 40 °C/75% RH  than at 25 °C /60% RH. No significant differences were found between the  initial and final pH value for the solution at the studied conditions. No  detectable changes in color, odor or precipitations were observed for the  solutions stored at the upper conditions. Conclusions: Bromhexine  hydrochloride 1% w/v oral solution could be formulated and remains stable  for at least 2 years when is stored at 25°C /60% RH and for 16 months when  stored at 40 °C /75% RH.  

This study was aimed to develop valsartan/ hydrochlorothiazide tablet formulation and to develop a stability indicating HPLC method for their analysis in raw materials and in its final dosage form according to the ICH guidelines. Film coating tablets containing valsartan and hydrochlorothiazide were developed. A gradient HPLC method was performed; the flow rate was 1.5 ml/min, injected volume 20μL, the mobile phases consist of two solvent: Solvent A (0.20 M ammonium acetate, adjusted to pH 5.6 with glacial acetic acid) and Solvent B (acetonitrile) and UV detection was carried out at 265nm. Valsartan and hydrochlorothiazide and their combined dosage form were exposed to thermal, oxidative, acid-base hydrolytic stress conditions, the stressed samples were analyzed. The method was validated with respect to linearity, precision, accuracy, system suitability, and robustness. The used method is specific for the estimation of valsartan and hydrochlorothiazide in presence of their degradation products and impurities. The method was linear over the range of 2.5–32μg/mL and 17.5-224μg/mL for valsartan and hydrochlorothiazide respectively. The mean recoveries were 100±2% for valsartan and hydrochlorothiazide respectively. The percentage of relative standard deviation (%RSD) was found to be less than critical value. Our developed analytical method is a stability indicating, economical and easy method which is useful in the quality control of valsartan and hydrochlorothiazide in tablet dosage forms.

Purpose: The aim of this study is to evaluate the chemical stability of Povidone-Iodine (PVP-I) after its incorporation into commercial cleaning formulations. Method: PVP-I was incorporated into five different trademarks of cleaning formulation designated F1through F5. Chemical stability of PVP-Ion months 0,1,2,3 and 4 was checked. pH, color, smell, and foam properties of the obtained cleaning formulations were tested. Results: PVP-I in Shampoo (F1) and hair & body wash (F2) was stable for more than 4 months, while in the shower gel (F3) and liquid soap (F4) was stable within the limit period of three month. The body wash (F5) was stable for only one month. The pH of the formulations remains constant during the entire period of the study. The foam volume and stability was comparable to that obtained from the starting trademarks. No change in smell or color of the cleaning formulations was observed. No sign of precipitate was observed within the entire period of study. Conclusion: Compounding PVP-I into cleaning trademarks in order to achieve disinfection and medical benefits can be followed. Cleaner industries should take seriously in consideration this issue to improve their selling.  

Diminazene aceturate and Antipyrine combination therapy is widely used in veterinary medicine. A simple reverse HPLC method for the analysis of samples of a ready injectable formulation containing a mixture of active ingredients and inactive excipients has been developed. The HPLC analysis was carried out using a reversed phase (RP)-C18 (250 mm×4.0 mm, 5 μm) column. The isocratic mobile phase consisted of a mixture of acetonitrile, methanol, phosphate buffer and hexane sulfonate; the flow rate was 0.6 mL/min and ultraviolet detection was at 291 nm. This method was validated in accordance with FDA and ICH guidelines and showed good linearity, accuracy, precision, selectivity and the system suitability results were within the acceptance criteria. A stability-indicating study was also carried out and indicated that this method could be used for purity and degradation evaluation of these formulations.