This study has been carried out to evaluate the possible In vitro interaction between ethanolic extracts of Rus coriaria (seed), Sacropoterium spinosum (seed) and Rosa damascena (flower) and certain known antimicrobial drugs including oxytetracycline HCl, penicillin G, cephalexin, sulfadimethoxine as sodium and enrofloxacin. Synergy testing of these extracts and antibiotics was carried out against 3 multidrug-resistant Pseudomonas aeruginosa strains using microdilution method. Results of this study showed that there is a decrease in the MIC in case of combination between ethanolic plant extracts and test antimicrobial agents. The most interesting result that the combination between R. coriaria and these antibiotics, which showed a high decrease in MIC and a strong bactericidal activity against these strains. These results may indicate that combinations between R. coriaria extract and these antibiotics could be useful in fighting emerging drug-resistance P. aeruginosa, this may due to that R. coriaria extract contain natural inhibitors working by different mechanisms or inhibiting efflux pumps. Now we have experiments underway leading to the identification of the active molecules present in R. coriaria. Further, in vivo experiments are needed to confirm Pseudomonal protection
This study has been carried out to evaluate the possible In vitro interaction between ethanolic extracts of Rus coriaria (seed), Sacropoterium spinosum (seed) and Rosa damascena (flower) and certain known antimicrobial drugs including oxytetracycline HCl, penicillin G, cephalexin, sulfadimethoxine as sodium and enrofloxacin. Synergy testing of these extracts and antibiotics was carried out against 3 multidrug-resistant Pseudomonas aeruginosa strains using microdilution method. Results of this study showed that there is a decrease in the MIC in case of combination between ethanolic plant extracts and test antimicrobial agents. The most interesting result that the combination between R. coriaria and these antibiotics, which showed a high decrease in MIC and a strong bactericidal activity against these strains. These results may indicate that combinations between R. coriaria extract and these antibiotics could be useful in fighting emerging drug-resistance P. aeruginosa, this may due to that R. coriaria extract contain natural inhibitors working by different mechanisms or inhibiting efflux pumps. Now we have experiments underway leading to th e identification of the active molecules present in R. coriaria. Further, in vivo experiments are needed to confirm Pseudomonal protection.
Objectives: This study has been done to evaluate the interaction between
ethanolic extracts of Rhus coriaria (seed), Psidium guajava (Leaf), Lawsonia
inermis (Leaf) and Sacropoterium spinosum (seed) and antimicrobial drugs
including oxytetracycline HCl, enrofloxacin, gentamicin sulphate and
sulfadimethoxine against four clinical isolates of methicillin-resistant
Staphylococcus aureus (MRSA).
Methodology: Evaluation of the interaction
between ethanolic extracts and different antimicrobial agents has been done
using well-diffusion method. Results: It showed that ethanolic extracts
increase the inhibition zones of oxytetracycline HCl, gentamicin sulphate, and
sulfadimethoxine, while combinations between these plant extracts and
enrofloxacin decrease inhibition zone.
Conclusion: This study probably suggests
the possibility of concurrent use of these antimicrobial drugs and plant
extracts in combination in treating infections caused by S. aureus strains or
at least the concomitant administration may not impair the antimicrobial
activity of these antibiotics.
Objective To evaluate the possible in vitro
interaction between ethanolic extracts of Rhus coriaria (R. coriaria)
(seed), Sacropoterium spinosum (S. spinosum) (seed), Rosa
damascena (R. damascene) (flower) and certain known antimicrobial
drugs including oxytetracycline HCl, penicillin G, cephalexin, sulfadimethoxine
as sodium, and enrofloxacin. This synergy study was carried out against 3
clinical strains of multidrug-resistant Pseudomonas aeruginosa (P.
aeruginosa).
Methods Evaluation of synergy interaction between plant extracts and
antimicrobial agents was carried out using microdilution method.
Results The results of this study showed that there is a decrease in the
MIC in case of combination of ethanolic plant extracts and test antimicrobial
agents. The most interesting result was that the combination between R.
coriaria and these antibiotics, showed a high decrease in minimum
inhibitory concentration (MIC), and a strong bactericidal activity against
these strains.
Conclusions These results may indicate that combinations between R.
coriaria extract and these antibiotics could be useful in fighting emerging
drug-resistance P. aeruginosa, which may due to that R. coriaria
extract contain natural inhibitors working by different mechanisms or
inhibiting efflux pumps. Now we have experiments underway leading to the
identification of the active molecules present in R. coriaria. Further,
in vivo experiments are needed to confirm pseudomonal protection.
Objective To evaluate the antimicrobial
activity of ethanolic extract of Ecballium elaterium (E. elaterium)
fruits alone against Staphylococcus aureus (S. aureus) strains
and Candida albicans (C. albicans) strains, or in combination
with penicillin against Staphylococcus areus strains.
Methods Evaluation of the antimicrobial activity or synergy interaction
was carried out using microdilution method.
Results The results showed that ethanolic extract of E. elaterium
fruits has antimicrobial activity against methicillin resistant S. aureus
(MRSA), methicillin sensitive S. aureus (MSSA) and C. albicans.
This extract showed a significant decrease in minimum inhibitory concentrations
(MIC) of penicillin against both MRSA and MSSA strains. Fractional inhibitory
concentration index (FIC) between penicillin and ethanolic extract of E.
elaterium fruits against these test strains was less than 0.5.
Conclusions This study suggests that ethanolic extract of E.
elaterium fruits has antimicrobial activity against S. aureus and C.
albicans and there is a possibility of concurrent use of penicillin and E.
elaterium extract in combination in the treatment of infections caused by
MRSA and MSSA strains. A wider study is needed to identify the effective
components, the mode of action and the possible toxic effect in vivo of
these ingredients.
Laboratory experiments were carried out in order to assess the insecticidal effect of Metarhizium anisopliae (Metschinkoff) Sorokin (Deuteromycotina: Hyphomycetes) formulations against larvae of Tribolium confusum Du Val (Coleoptera: Tenebrionidae) on flour and wheat. M. anisopliae was applied at three dose rates, of 8106 , 8108 and 81010 conidia/kg to either wheat or flour, respectively. Also the wheat/flour was treated with the diatomaceous earth (DE) formulation SilicoSec (Biofa, Germany), at two dose rates, 0.2 and 0.5 g/kg of wheat or flour either alone or in combination withM. anisopliae with each fungal rate. Mortality ofT. confusum larvae was assessed 7 days after exposure to the treated substrate. The bioassays were conducted at three temperatures, 20, 25 and 301C, and two relative humidity (r.h.) levels, 55% and 75%. Larval mortality was notably varied among treatments, as well among temperature and humidity levels. For both fungus and DE, the increase of temperature increased their effectiveness. On the other hand, the increase of r.h. significantly reduced larval mortality for both M. anisopliae and SilicoSec. Both substances, either alone or in combination, were more effective on wheat than on flour. Also, the addition of 0.5 g of SilicoSec in the fungal preparation, especially at the highest dose rate, increased larval mortality, in comparison with the fungus alone or in combination with 0.2 g of SilicoSec. The results of the present work suggest that, under certain circumstances, the effectiveness of M. anisopliae against T. confusum larvae can be benefitted by the presence of DE.
Objective To evaluate the antimicrobial activity of ethanolic extract of Ecballium elaterium (E. elaterium) fruits alone against Staphylococcus aureus (S. aureus) strains and Candida albicans (C. albicans) strains, or in combination with penicillin against Staphylococcus areus strains. Methods Evaluation of the antimicrobial activity or synergy interaction was carried out using microdilution method.
Results The results showed that ethanolic extract of E. elaterium fruits has antimicrobial activity against methicillin resistant S. aureus (MRSA), methicillin sensitive S. aureus (MSSA) and C. albicans. This extract showed a significant decrease in minimum inhibitory concentrations (MIC) of penicillin against both MRSA and MSSA strains. Fractional inhibitory concentration index (FIC) between penicillin and ethanolic extract of E. elaterium fruits against these test strains was less than 0.5.
Conclusions This study suggests that ethanolic extract of E. elaterium fruits has antimicrobial activity against S. aureus and C. albicans and there is a possibility of concurrent use of penicillin and E. elaterium extract in combination in the treatment of infections caused by MRSA and MSSA strains. A wider study is needed to identify the effective components, the mode of action and the possible toxic effect in vivo of these ingredients.