Objectives: This study has been done to evaluate the interaction between
ethanolic extracts of Rhus coriaria (seed), Psidium guajava (Leaf), Lawsonia
inermis (Leaf) and Sacropoterium spinosum (seed) and antimicrobial drugs
including oxytetracycline HCl, enrofloxacin, gentamicin sulphate and
sulfadimethoxine against four clinical isolates of methicillin-resistant
Staphylococcus aureus (MRSA).
Methodology: Evaluation of the interaction
between ethanolic extracts and different antimicrobial agents has been done
using well-diffusion method. Results: It showed that ethanolic extracts
increase the inhibition zones of oxytetracycline HCl, gentamicin sulphate, and
sulfadimethoxine, while combinations between these plant extracts and
enrofloxacin decrease inhibition zone.
Conclusion: This study probably suggests
the possibility of concurrent use of these antimicrobial drugs and plant
extracts in combination in treating infections caused by S. aureus strains or
at least the concomitant administration may not impair the antimicrobial
activity of these antibiotics.
Mycoparasitic Pythium species with spiny oogonia were surveyed in 50 Palestinian agricultural fields subject to different cropping practices using the Sclerotia Bait Technique (SBT) and the Surface-Soil-Dilution-Plate method (SSDP) with the selective VP3 medium. The mycoparasitic Pythium species were obtained from 21 (42%) soils using the SSDP method and from 37 (74%) soils using SBT. Pythium acanthicum and P. oligandrum were isolated by both methods, whereas P. periplocum was isolated only by the SBT. Using a newly modified dual plate culture method (MDPCM), the three mycoparasites showed varying antagonistic performance against several Pythium host species under a range of in vitro conditions. However, P. periplocum and P. oligandrum were found to be active biocontrol agents against P. ultimum, the damping-off organism of cucumber. This pathogen was antagonized, on thin films of water agar, by the three mycoparasites, and was moderately susceptible to P. periplocum while slightly susceptible to P. acanthicum and P. oligandrum. In direct application method in which antagonistic mycoparasites were incorporated into peat/sand mixture artificially infested with P. ultimum under growthroom conditions, Pythium oligandrum and P. periplocum (at 500 CFUg-1) significantly improved seedling emergence and protected seedlings from damping-off. In the seed coating method, biocontrol by two types of seed dressing (homogenateor oospore coated seeds), was comparable to that achieved by direct application.
Objectives: This study has been done to evaluate the interaction between ethanolic extracts of Rhus coriaria (seed), Psidium guajava (Leaf), Lawsonia inermis (Leaf) and Sacropoterium spinosum (seed) and antimicrobial drugs including oxytetracycline HCl, enrofloxacin, gentamicin sulphate and sulfadimethoxine against four clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA).
Methodology: Evaluation of the interaction between ethanolic extracts and different antimicrobial agents has been done using well-diffusion method.
Results: It showed that ethanolic extracts increase the inhibition zones of oxytetracycline HCl, gentamicin sulphate, and sulfadimethoxine, while combinations between these plant extracts and enrofloxacin decrease inhibition zone.
Conclusion: This study probably suggests the possibility of concurrent use of these antimicrobial drugs and plant extracts in combination in treating infections caused by S. aureus strains or at least the concomitant administration may not impair the antimicrobial activity of these antibiotics.