antagonism

adwank's picture

In Vitro Activity of Certain Drugs in Combination with Plant Extracts against Staphylococcus Aureus Infections

Journal Title, Volume, Page: 
PAKISTAN JOURNAL OF MEDICAL SCIENCES, Volume: 24 Issue: 4 Pages: 541-544 Published: JUL-SEP 2008
Year of Publication: 
2008
Authors: 
Ghaleb Mohamma Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Bassam Ali Abu-shanab
Faculty of Veterinary Medicine, An-Najah N. University, Nablus, Palestine
Kamel Mohammad Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Preferred Abstract (Original): 

Objectives: This study has been done to evaluate the interaction between ethanolic extracts of Rhus coriaria (seed), Psidium guajava (Leaf), Lawsonia inermis (Leaf) and Sacropoterium spinosum (seed) and antimicrobial drugs including oxytetracycline HCl, enrofloxacin, gentamicin sulphate and sulfadimethoxine against four clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA).
Methodology: Evaluation of the interaction between ethanolic extracts and different antimicrobial agents has been done using well-diffusion method. Results: It showed that ethanolic extracts increase the inhibition zones of oxytetracycline HCl, gentamicin sulphate, and sulfadimethoxine, while combinations between these plant extracts and enrofloxacin decrease inhibition zone.
Conclusion: This study probably suggests the possibility of concurrent use of these antimicrobial drugs and plant extracts in combination in treating infections caused by S. aureus strains or at least the concomitant administration may not impair the antimicrobial activity of these antibiotics. 

bassamas's picture

In Vitro Activity of Certain Drugs in combination with Plant Extracts Against staphylococcus Aureus Infections

Journal Title, Volume, Page: 
Pak J Med Sci, Vol. 24 No. 4
Year of Publication: 
2008
Authors: 
Bassam Ali Abu-Shanab
Faculty of Veterinary Medicine, An-Najah N. University, Nablus, PALESTINE
Current Affiliation: 
Department of Animal Production and Health, Faculty of Agriculture and Veterinary Medicine, An Najah National University, Nablus, Palestine
Ghaleb Mohammad Adwan
Department of Biology and Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus
Kamel Mohammad Adwan
Department of Biology and Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus
Preferred Abstract (Original): 
Objectives: This study has been done to evaluate the interaction between ethanolic extracts ofRhus coriaria (seed) , Psidium guajava (Leaf), Lawsonia inermis (Leaf) and Sacropoterium spinosum(seed) and antimicrobial drugs including oxytetracycline HCl, enrofloxacin, gentamicin sulphateand sulfadimethoxine against four clinical isolates of methicillin-resistant Staphylococcus aureus(MRSA).Methodology: Evaluation of the interaction between ethanolic extracts and differentantimicrobial agents has been done using well-diffusion method.Results: It showed that ethanolic extracts increase the inhibition zones of oxytetracycline HCl,gentamicin sulphate, and sulfadimethoxine, while combinations between these plant extractsand enrofloxacin decrease inhibition zone.Conclusion: This study probably suggests the possibility of concurrent use of these antimicrobialdrugs and plant extracts in combination in treating infections caused by S. aureus strains or atleast the concomitant administration may not impair the antimicrobial activity of theseantibiotics.
MSShtayeh's picture

Isolation of Pythium Acanthicum, P. Oligandrum, And P. Periplocum From Soil And Evaluation of Their Mycoparasitic Activity And Biocontrol Efficacy Against Selected Phytopathogenic Pythium Species

Journal Title, Volume, Page: 
Mycopathologia Volume 145, Number 3, 143-153
Year of Publication: 
1999
Authors: 
Mohammed S. Ali-Shtayeh
Department of Biology , Faculty of Science, An-Najah National University, Nablus. Palestine
Current Affiliation: 
Department of Biology , Faculty of Science, An-Najah National University, Nablus. Palestine
Amjed S. F. Saleh
Department of Biology, An-Najah University, Nablus, P.O. Box 696, Palestinian Autonomous Territory
Preferred Abstract (Original): 

Mycoparasitic Pythium species with spiny oogonia were surveyed in 50 Palestinian agricultural fields subject to different cropping practices using the Sclerotia Bait Technique (SBT) and the Surface-Soil-Dilution-Plate method (SSDP) with the selective VP3 medium. The mycoparasitic Pythium species were obtained from 21 (42%) soils using the SSDP method and from 37 (74%) soils using SBT. Pythium acanthicum and P. oligandrum were isolated by both methods, whereas P. periplocum was isolated only by the SBT. Using a newly modified dual plate culture method (MDPCM), the three mycoparasites showed varying antagonistic performance against several Pythium host species under a range of in vitro conditions. However, P. periplocum and P. oligandrum were found to be active biocontrol agents against P. ultimum, the damping-off organism of cucumber. This pathogen was antagonized, on thin films of water agar, by the three mycoparasites, and was moderately susceptible to P. periplocum while slightly susceptible to P. acanthicum and P. oligandrum. In direct application method in which antagonistic mycoparasites were incorporated into peat/sand mixture artificially infested with P. ultimum under growthroom conditions, Pythium oligandrum and P. periplocum (at 500 CFUg-1) significantly improved seedling emergence and protected seedlings from damping-off. In the seed coating method, biocontrol by two types of seed dressing (homogenateor oospore coated seeds), was comparable to that achieved by direct application.

adwang's picture

In Vitro Activity of Certain Drugs In Combination With Plant Extracts Against Staphylococcus Aureus Infections

Journal Title, Volume, Page: 
Pak J Med Sci July - September 2008 Vol. 24 No. 4 541-544
Year of Publication: 
2008
Authors: 
Ghaleb Mohammad Adwan
Department of Biology And Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus Palestine
Current Affiliation: 
Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine
Bassam Ali Abu-Shanab
Faculty Of Veterinary Medicine, An-Najah N. University, Nablus, Palestine
Kamel Mohammad Adwan
Department of Biology and Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus, Palestine.
Preferred Abstract (Original): 

Objectives: This study has been done to evaluate the interaction between ethanolic extracts of Rhus coriaria (seed), Psidium guajava (Leaf), Lawsonia inermis (Leaf) and Sacropoterium spinosum (seed) and antimicrobial drugs including oxytetracycline HCl, enrofloxacin, gentamicin sulphate and sulfadimethoxine against four clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA).
Methodology: Evaluation of the interaction between ethanolic extracts and different antimicrobial agents has been done using well-diffusion method.
Results: It showed that ethanolic extracts increase the inhibition zones of oxytetracycline HCl, gentamicin sulphate, and sulfadimethoxine, while combinations between these plant extracts and enrofloxacin decrease inhibition zone.
Conclusion: This study probably suggests the possibility of concurrent use of these antimicrobial drugs and plant extracts in combination in treating infections caused by S. aureus strains or at least the concomitant administration may not impair the antimicrobial activity of these antibiotics.

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