An-Najah Staff

This study aimed to evaluate antibacterial and antifungal activities and exhaustive extraction yields of the aqueous and organic extracts of Verbascum sinuatum L., against possible human pathogens, which are the fungus Candida albicans, gram positive bacteria Bacillus subtilis, Staphylococcus aureus Staphylococcus epidermidis and the gram negative bacteria Eschrichia coli and Pseudomonas aeruginosa. Well diffusion method was used in screening antimicrobial activity for the plant extracts, in which the diameters of inhibition zones were measured and compared to a positive control. Serial dilution method was used for measuring the minimum inhibition concentrations for each microorganism. In well diffusion method, the plant’s aqueous extract has antimicrobial activity to all the tested organisms except Pseudomonas aeruginosa and Candida albicans, with variable diameters of inhibition zone. The percent inhibition compared to the positive control imipenem was 39.13% for Staphylococcus aureus, 37.5% for Staphylococcus epidermidis, Eschrichia coli for 30.55% and the least 30.43% for Bacillus subtilis. The organic extract exhibited inhibition activity 26.08% against Bacillus subtilis and 50% against Eschrichia coli. In the serial dilution method, the aqueous extract exhibited inhibition for all the test microorganisms. At initial concentration of 20 mg/ml, the lowest MIC value was for Staphylococcus aureus 1.28 µg/ml, and highest for Staphylococcus epidermidis 4000 µg/ml. The MIC values for Pseudomonas aeruginosa 160 µg/ml, 800 µg/ml for Bacillus subtilis, 800 µg/ml for Eschrichia coli and 32 µg/ml for Candida albicans respectively. This study showed that V. sinuatum extract has a broad spectrum activity against gram positive and gram negative bacteria, as well as anticandidal activity.

The antibacterial effects of single and combined plant extracts of water, ethanol and methanol for two nutraceuticals utilized in Palestine were studied against multiple drug resistances Pseudomonas aeruginosa using well diffusion method. These plants are Rhus coriaria and Thymus vulgaris. Combinations of these extracts showed an additive action against this pathogen.

Objective To evaluate the possible in vitro interaction between ethanolic extracts of Rhus coriaria (R. coriaria) (seed), Sacropoterium spinosum (S. spinosum) (seed), Rosa damascena (R. damascene) (flower) and certain known antimicrobial drugs including oxytetracycline HCl, penicillin G, cephalexin, sulfadimethoxine as sodium, and enrofloxacin. This synergy study was carried out against 3 clinical strains of multidrug-resistant Pseudomonas aeruginosa (P. aeruginosa).
Methods Evaluation of synergy interaction between plant extracts and antimicrobial agents was carried out using microdilution method.
Results The results of this study showed that there is a decrease in the MIC in case of combination of ethanolic plant extracts and test antimicrobial agents. The most interesting result was that the combination between R. coriaria and these antibiotics, showed a high decrease in minimum inhibitory concentration (MIC), and a strong bactericidal activity against these strains.
Conclusions These results may indicate that combinations between R. coriaria extract and these antibiotics could be useful in fighting emerging drug-resistance P. aeruginosa, which may due to that R. coriaria extract contain natural inhibitors working by different mechanisms or inhibiting efflux pumps. Now we have experiments underway leading to the identification of the active molecules present in R. coriaria. Further, in vivo experiments are needed to confirm pseudomonal protection.

The antibacterial effects of single and combined plant extracts of water, ethanol and methanol for two nutraceuticals utilized in Palestine were studied against multiple drug resistances Pseudomonas aeruginosa using well diffusion method. These plants are Rhus coriaria and Thymus vulgaris. Combinations of these extracts showed an additive action against this pathogen.

Objective To evaluate the possible in vitro interaction between ethanolic extracts of Rhus coriaria (R. coriaria) (seed), Sacropoterium spinosum (S. spinosum) (seed), Rosa damascena (R. damascene) (flower) and certain known antimicrobial drugs including oxytetracycline HCl, penicillin G, cephalexin, sulfadimethoxine as sodium, and enrofloxacin. This synergy study was carried out against 3 clinical strains of multidrug-resistant Pseudomonas aeruginosa (P. aeruginosa). Methods Evaluation of synergy interaction between plant extracts and antimicrobial agents was carried out using microdilution method. Results The results of this study showed that there is a decrease in the MIC in case of combination of ethanolic plant extracts and test antimicrobial agents. The most interesting result was that the combination between R. coriaria and these antibiotics, showed a high decrease in minimum inhibitory concentration (MIC), and a strong bactericidal activity against these strains. Conclusions these results may indicate that combinations between R. coriaria extract and these antibiotics could be useful in fighting emerging drug-resistance P. aeruginosa, which may due to that R. coriaria extract contain natural inhibitors working by different mechanisms or inhibiting efflux pumps. Now we have experiments underway leading to the identification of the active molecules present in R. coriaria. Further, in vivo experiments are needed to confirm pseudomonal protection.

Objective: To evaluate the possible in vitro interaction between ethanolic extracts of Rhus coriaria (R. coriaria) (seed), Sacropoterium spinosum (S. spinosum) (seed), Rosa damascena (R.damascene) (flower) and certain known antimicrobial drugs including oxytetracycline HCl,pweansi ccialrlirnie Gd ,o cuetp ahgaaleinxsint ,3 s ucllfiandicimale sthtroaxiinnse oafs msoudltiiudmru, ga-nrde seinstraonflto xPasceiund. oTmhoisn assy naeerrguyg sintuodsay (P. aeruginosa).
Methods: Evaluation of synergy interaction between plant extracts and antimicrobial agents was carried out using microdilution method.
Results: The results of this satnudd yte ssht oawnetdim thicarto tbhiearle a igse an tdse. cTrehaes em ions tt hien tMerIeCs tiinn gc arsees uolft cwomasb tihnaatt iothne o cf oemthbainnoaltiico np labnett wexeterna cRts.coriaria and these antibiotics, showed a high decrease in minimum inhibitory concentration (MIC),and a strong bactericidal activity against these strains.
Conclusions: These results may indicate that combinations between R. coriaria extract and these antibiotics could be useful in fighting emerging drug-resistance P. aeruginosa, which may due to that R. coriaria extract contain natural iunnhdiebriwtoarys wleoardkiinngg tboy t hdeif fiedreenntti fmiceacthioann iosfm tsh eo ra icnthivieb imtinogle ecfuflluesx ppruemsepnst. Nino wR . wceo rhiaarviea .e xFpuerrthimere,n itns vivo experiments are needed to confirm pseudomonal protection.

The antibacterial effects of single and combined plant extracts of water, ethanol and methanol for two nutraceuticals utilized in Palestine were studied against multiple drug resistances Pseudomonas aeruginosa using well diffusion method. These plants are Rhus coriaria and Thymus vulgaris. Combinations of these extracts showed an additive action against this pathogen.