Total phenols
Preliminary Phytochemical Screening, Quantitative Estimation of Total Flavonoids, Total Phenols and Antioxidant Activity of Ephedra alata Decne
Thu, 2016-02-04 15:44 — Nidal Amin JaradatPhytochemical analyses indicated the presence of cardiac glycosides, reducing sugars, flavonoids, phenolic compounds and alkaloids. The total phenolic content in the ethanolic extract was 19.175 mg gallic acid while was the highest in the methanolic extract which was 47.62 mg gallic acid equivalent/g of extract powder. The total flavonoid content of the plant was 0.519 mg RU/g in the aqueous extract and 5.44 mg RU/g in the ethanolic extract while was the highest in the methanolic extract 54.66 mg RU/g.
In the same time the E. alata methanolic extract showed that it has high antioxidant activity and powerful oxygen free radical scavenging abilities as well as the IC50 for the plant was almost equivalent to the Trolox standard antioxidant which justified its uses in the Palestinian traditional medicines and could represented as a good candidate for further biological and chemical analysis, and can be further subjected for isolation of the therapeutically active compounds with anticancer activity and also for further pharmacological evaluations.
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TwitterPhytoconstituents, Free Radical Scavenging Potential, Total Phenols and Total Flavonoids Assessments for Violet Horned Poppy from Jerusalem Mountains
Mon, 2016-02-01 11:53 — Nidal Amin JaradatPhytochemical Analysis, Quantitative Estimations of Total Phenols and Free Radical Scavenging Activity of Bupleurum subovatum from Jerusalem
Mon, 2016-02-01 11:43 — Nidal Amin JaradatThe crude extracts from Bupleurum subovatum plant were used to screen the presence of secondary metabolic products, to estimate the total phenol content and free radical scavenging activity for the plant extracts. Methods:Antioxidant activity was evaluated by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay, while total phenol content was determined by using Folin Ciocalteu' s method. Result:The phytochemical analysis confirmed the presence of phenol, proteins, starch, reducing sugars, tannins, volatile oils, cardiac glycosides, steroid, and huge amounts of saponins. Total phenolic content in the methanolic extract was 9.05 mg/g Gallic acid. At the same time, methanolic extract showed a mild potential oxygen free radical scavenging ability as well as the IC50 for the plant was 18.60 ± 0.36 µg/ml, which justified its uses in the folkloric medicine and could be further subjected for the isolation of their therapeutic active compounds. Conclusion:The results of this study revealed the antioxidant activity and confirmed the therapeutic usage of Bupleurum subovatum in the traditional medicine.
In Vitro Antioxidant and Antitumor Activities of Six Selected Plants Used in the Traditional Arabic Palestinian Herbal Medicine
Sun, 2014-09-28 13:26 — Dr. Waheed J. JondiContext: Despite several pharmacological applications
of the medicinal plants in the Traditional Arabic Palestinian Herbal Medicine
in Palestine (TAPHM), studies on their antioxidant properties are still scarce.
Objective: This work evaluates the antioxidant and antitumor activities
of the ethanol extracts from different parts of six plants: [Arum
palaestinum Boiss (Araceae), Urtica pilulifera L. (Urticaceae),
Coridothymus capitatus (L.) Reichb (Lamiaceae), Majorana syriaca
(L.) Rafin. (Lamiaceae), Teucrium creticum L. (Lamiaceae), and Teucrium
capitatum L. (Lamiaceae)] used in the TAPHM.
Materials and methods: The antioxidant activity was evaluated for the
ethanol extracts by DPPH and β-carotene–linoleic acid assays together with
total contents of phenols and flavonoids. For the anti-carcinogenic evaluation,
the extracts were tested for the ability to inhibit the proliferation of breast
cancer cells (MCF-7) using the MTT reduction assay.
Results: Among the extracts, the U. pilulifera had the highest
amount of total phenolics, possessing the second highest total flavonoids. It
also showed a maximum cytotoxic activity (IC50 = 63 µg/ml), followed
by C. capitatus, and A. palaestinum. Otherwise, the extract of T.
creticum was demonstrated to be an efficient scavenger of O2 (IC50 = 83 µg/ml),
followed by M. syriaca, C. capitatus, T. capitatum, A. palaestinum, and U.
pilulifera.
Discussion and conclusion: The results suggest that the investigated
plants have shown varied antioxidant capacities which were strongly correlated
with their contents of phenolics. Accordingly, this study proposes that the
therapeutic benefit of these plants can be, at least in part, attributed to its
potential inhibition of oxidative processes.
In Vitro Antioxidant and Antitumor Activities of Six Selected Plants Used In the Traditional Arabic Palestinian Herbal Medicine
Wed, 2014-02-19 12:11 — Mohammed Saleem Ali-ShtayehContext: Despite several pharmacological applications of the medicinal
plants in the Traditional Arabic Palestinian Herbal Medicine in
Palestine (TAPHM), studies on their antioxidant properties are yet
scarce.
Objective: This work evaluates the antioxidant and anti-tumor activities
of the ethanol extracts from different parts of six plants: (Arum
palaestinum Boiss (Araceae), Urtica pilulifera L. (Urticaceae),
Coridothymus capitatus (L.) Reichb ( Lamiaceae), Majorana syriaca (L.)
Rafin. (Lamiaceae), Teucrium creticum L. (Lamiaceae), Teucrium capitatum
L. (Lamiaceae), used in the TAPHM.
Materials and methods: The antioxidant activity was evaluated for the
ethanol extracts by DPPH and β-carotene-linoleic acid assays together
with total contents of phenols and flavonoids. For the anti-carcinogenic
evaluation, the extracts were tested for the ability to inhibit the
proliferation of breast cancer cells (MCF-7) using the MTT reduction
assay
Results: Among the extracts, the U. pilulifera had the highest amount of
total phenolics, possessing the second highest total flavonoids. It
also showed a maximum cytotoxic activity (IC50 = 63 µg/ ml), followed by
C. capitatus, and A. palaestinum. Otherwise, the extract of T. creticum
has demonstrated to be an efficient scavenger of O2 (IC50= 90 µg/ml),
followed by C. capitatus, T. capitatum, A. palaestinum, and U.
pilulifera.
Discussion and conclusion: The results suggest that the investigated
plants have shown varied antioxidant capacities which were strongly
correlated with their contents of phenolics. Accordingly, this study
proposes that the therapeutic benefit of these plants can be, at least
in part, attributed to its potential inhibition of oxidative processes.
