Phytochemical screening

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Preliminary Phytochemical Screening, Quantitative Estimation of Total Flavonoids, Total Phenols and Antioxidant Activity of Ephedra alata Decne

Journal Title, Volume, Page: 
Journal of Materials and Environmental Science 6(6):1771-1778 · June 2015
Year of Publication: 
2015
Authors: 
Nidal Amin Jaradat
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Fatima Mohammed Hussen
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Anas Al Ali
Department of Chemistry, Faculty of Science, An-Najah National University, P.O. Box 7, Nablus, Palestine
Preferred Abstract (Original): 
This study designed to evaluate antioxidant activity, screen the existence of phytogenic chemical compounds and to determine the total flavonoid and phenol contents of the Ephedra alata. to prove its utilization in the Palestinian folk medicine for treatment of cancer. Total flavonoid contents of the plant were determined by using rutin reference standard method and total phenols determined by using Folin Ciocalteu method while antioxidant activity evaluated by using 2, 2-diphenyl-1-picryl-hydrazyl-hydrate assay.
Phytochemical analyses indicated the presence of cardiac glycosides, reducing sugars, flavonoids, phenolic compounds and alkaloids. The total phenolic content in the ethanolic extract was 19.175 mg gallic acid while was the highest in the methanolic extract which was 47.62 mg gallic acid equivalent/g of extract powder. The total flavonoid content of the plant was 0.519 mg RU/g in the aqueous extract and 5.44 mg RU/g in the ethanolic extract while was the highest in the methanolic extract 54.66 mg RU/g.
In the same time the E. alata methanolic extract showed that it has high antioxidant activity and powerful oxygen free radical scavenging abilities as well as the IC50 for the plant was almost equivalent to the Trolox standard antioxidant which justified its uses in the Palestinian traditional medicines and could represented as a good candidate for further biological and chemical analysis, and can be further subjected for isolation of the therapeutically active compounds with anticancer activity and also for further pharmacological evaluations.
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Phytoconstituents, Free Radical Scavenging Potential, Total Phenols and Total Flavonoids Assessments for Violet Horned Poppy from Jerusalem Mountains

Journal Title, Volume, Page: 
J. Mater. Environ. Sci. 6 (10) (2015) 2958-2966 ISSN : 2028-2508 CODEN: JMESCN
Year of Publication: 
2015
Authors: 
Nidal Amin Jaradat
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Fatima Mohammed Hussen
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Anas Al Ali
Department of Chemistry, Faculty of Science, An-Najah National University, P.O. Box 7, Nablus, Palestine
Hasan Y. Alniss
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Maram Dweikat
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Preferred Abstract (Original): 
Most of the world population especially in the developing countries used herbal medicaments due to their cultural acceptability, safety, efficacy and fewer side effects for their primary health care. Dearth in the studies related to Roemeria hybrida has prompted us to do the present work. This study was designed to detect the presence of bioactive compounds and to evaluate total phenols, total flavonoids contents and antioxidant activity of Roemeria hybrida plant growing in the mountains of Jerusalem/ Palestine. The results of such a study would be important to prove or disprove its utilization in the Palestinian folk medicine for treatment of retinopathy (diabetes mellitus) and other diseases. Total flavonoid contents were determined by using Rutin reference standard method and total phenols determined by using Folin Ciocalteu’s method while antioxidant activity evaluated by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. The results showed that the aqueous extract contains carbohydrate, reducing sugars, glycosides, flavonoids, terpenoids, phenols and tannin. The result also revealed that this plant has an antioxidant activity (IC50=31.405±0.21µg/ml), as well as total phenol was 30.448±0.56 mg of GA/g while the total flavonoid was 53.821 ±0.42 mg rutin/g of the plant extract. Therefore R. hybrida can be a good candidate for manufacturing of pharmacological active pharmaceuticals, cosmeceuticals and nutraceutical formulations.
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Phytochemical Analysis, Quantitative Estimations of Total Phenols and Free Radical Scavenging Activity of Bupleurum subovatum from Jerusalem

Journal Title, Volume, Page: 
Pharmaceutical Sciences 2015;21(4):205-21010.15171/PS.2015.38
Year of Publication: 
2016
Authors: 
Nidal Jaradat
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Ahmad Eid
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Fatima Abdelwahab
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Linda Isa
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Amjad Abdulrahman
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Murad Abualhasan
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Azmi Mahmoud Ali Hussein
Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Preferred Abstract (Original): 

The crude extracts from Bupleurum subovatum plant were used to screen the presence of secondary metabolic products, to estimate the total phenol content and free radical scavenging activity for the plant extracts.  Methods:Antioxidant activity was evaluated by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay, while total phenol content was determined by using Folin Ciocalteu' s method. Result:The phytochemical analysis confirmed the presence of phenol, proteins, starch, reducing sugars, tannins, volatile oils, cardiac glycosides, steroid, and huge amounts of saponins. Total phenolic content in the methanolic extract was 9.05 mg/g Gallic acid. At the same time, methanolic extract showed a mild potential oxygen free radical scavenging ability as well as the IC50 for the plant was 18.60 ± 0.36 µg/ml, which justified its uses in the folkloric medicine and could be further subjected for the isolation of their therapeutic active compounds. Conclusion:The results of this study revealed the antioxidant activity and confirmed the therapeutic usage of Bupleurum subovatum in the traditional medicine.

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