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Bupleurum subovatum

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Phytochemical Analysis, Quantitative Estimations of Total Phenols and Free Radical Scavenging Activity of Bupleurum subovatum from Jerusalem

Mon, 2016-02-01 11:43 — Nidal Amin Jaradat
Journal Title, Volume, Page: 
Pharmaceutical Sciences 2015;21(4):205-21010.15171/PS.2015.38
Year of Publication: 
2016
Link: 
http://journals.tbzmed.ac.ir/PHARM/Abstract/PHARM_3313_20150723151737
Authors: 
Nidal Jaradat
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
[email protected]
Ahmad Eid
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Fatima Abdelwahab
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Linda Isa
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Amjad Abdulrahman
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Murad Abualhasan
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Azmi Mahmoud Ali Hussein
Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Preferred Abstract (Original): 

The crude extracts from Bupleurum subovatum plant were used to screen the presence of secondary metabolic products, to estimate the total phenol content and free radical scavenging activity for the plant extracts.  Methods:Antioxidant activity was evaluated by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay, while total phenol content was determined by using Folin Ciocalteu' s method. Result:The phytochemical analysis confirmed the presence of phenol, proteins, starch, reducing sugars, tannins, volatile oils, cardiac glycosides, steroid, and huge amounts of saponins. Total phenolic content in the methanolic extract was 9.05 mg/g Gallic acid. At the same time, methanolic extract showed a mild potential oxygen free radical scavenging ability as well as the IC50 for the plant was 18.60 ± 0.36 µg/ml, which justified its uses in the folkloric medicine and could be further subjected for the isolation of their therapeutic active compounds. Conclusion:The results of this study revealed the antioxidant activity and confirmed the therapeutic usage of Bupleurum subovatum in the traditional medicine.

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