E. coli | An-Najah Staff

Natural dye-sensitized ZnO nano-particles as photo-catalysts in complete degradation of E. coli bacteria and their organic content

Sun, 2016-08-07 13:48 — Amani Lutfi Zubi

Journal Title, Volume, Page:

Journal of Photochemistry and Photobiology A: Chemistry , Volume 328, 1 September 2016, Pages 207–216

Year of Publication:

2016

Link:

http://www.sciencedirect.com/science/article/pii/S1010603016300119

Authors:

Ahed Zyoud

Current Affiliation:

SSERL, Chemistry, An-Najah National University, Nablus, Palestine

Majdi Dwikat

Current Affiliation:

Clinical Biology Lab, An-Najah National University, Nablus, Palestine

Samar Al-Shakhshir

Current Affiliation:

SSERL, Chemistry, An-Najah National University, Nablus, Palestine

Sondos Ateeq

Current Affiliation:

SSERL, Chemistry, An-Najah National University, Nablus, Palestine

Jumana Shteiwi

Current Affiliation:

SSERL, Chemistry, An-Najah National University, Nablus, Palestine

Amani Zu’bi

Current Affiliation:

SSERL, Chemistry, An-Najah National University, Nablus, Palestine

[email protected]

Muath H.S. Helal

Current Affiliation:

College of Pharmacy and Nutrition, E-wing Health Sciences, University of Saskatchewan, 104 Clinic Place, Saskatoon SK S7N 5E5, Canada

Guy Campet

Current Affiliation:

ICMCB, University of Bordeaux, Ave. Dr. A. Schweitzer, Bordeaux, France

DaeHoon Park

Current Affiliation:

Dansuk Industrial Co., LTD. #1239-5, Jeongwang-Dong, Shiheung-Si, Kyonggi-Do, 429-913, Republic of Korea

Hansang Kwon

Current Affiliation:

Department of Materials System Engineering, Pukyong National University, 365 Sinseonro, Namgu 608-739, Busan, Republic of Korea

Tae Woo Kim

Current Affiliation:

Energy Materials Laboratory, Korea Institute of Energy Research, 152 Gajeong-Ro, Yuseong-Gu, Daejeon City, 34129, Republic of Korea

Maher Kharoof

Current Affiliation:

Jerusalem Pharmaceutical Co., Nablus Street, Al-Bireh-Ramallah, Palestine

Ramzi Shawahna

Current Affiliation:

Department of Physiology, Pharmacology and Toxicology, College of Medicine and Health Sciences, An-Najah national University, Nablus, Palestine

Hikmat S. Hilal

Current Affiliation:

SSERL, Chemistry, An-Najah National University, Nablus, Palestine

[email protected]

Preferred Abstract (Original):

This communication describes for the first time how nano-size particles, sensitized with natural dye molecules of anthocyanin, can be used as catalysts in photo-degradation of gram negative Escherichia coli bacteria in water. The naked ZnO nano-particles degraded up to 83% of the bacteria under solar simulator light, while the dye-sensitized particles increased the bacterial loss by ∼10%. Solar simulator light includes about 5% of UV tail (shorter than 400 nm) which means that both UV and visible light (longer than 400 nm) radiations could be involved. When a cut-off filter was used, the naked ZnO caused only 40% bacterial loss, in accordance with earlier literature that described killing of bacteria with ZnO particles both in the dark and under light. With the cut-off filter, the sensitized ZnO particles caused higher than 90% bacterial loss, which confirms sensitization of the ZnO particles to visible light. Moreover, the results show that the catalyzed photo-degradation process causes mineralization of the bacteria and their organic internal components which leach out by killing. The catalyst can be recovered and reused losing ∼10% of its activity each time due to mineralization of the dye molecules. However, catalyst activity can be totally regained by re-sensitizing it with the anthocyanin dye. The effects of different experimental conditions, such as reaction temperature, pH, bacterial concentration and catalyst amount together with nutrient broth and saline media, will be discussed together with the role of the sensitizer.

Molecular Epidemiology of Antibiotic-Resistant Escherichia Coli Isolated From Hospitalized Patients with Urinary Tract Infections in Northern Palestine

Sun, 2014-03-16 14:57 — Nael Sodqi Abuhasan

Journal Title, Volume, Page:

Pol J Microbiol.;53(1):23-6 Year of Publication: 2004

Year of Publication:

2004

Link:

http://www.ncbi.nlm.nih.gov/pubmed/15330263

Authors:

Abu-Shanab B

Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine

Adwan K

Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine

Abu-Hasan N

Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine

Current Affiliation:

Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine

[email protected]

Adwan G

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Jarrar N.

Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine

Preferred Abstract (Original):

Eighty isolates of Escherichia coli were collected in Northern Palestine throughout the 1996 to 2000 period from hospitalized patients with urinary tract infections (UTIs). Resistance rates were ampicillin, 65%; co-trimoxazole, 55%; cefuroxime, 10%; cefotaxime, 7.5%; ceftazidime, 2.5%; ciprofloxacin, 12.5%; gentamicin, 6.25% and amikacin, 1.25%. No imipenem-resistant isolates were identified. To determine whether this was due to intra-hospital transmission of resistant strains, clonal structure of 10 multiple-resistant isolates was examined by genomic DNA fingerprinting by enterobacterial repetitive intergenic concensus-polymerase chain reaction (ERIC-PCR) and all were clonally distinct. Thus, these strains are likely resistant due to convergent acquisition of resistance determinants by genetically unrelated uropathogenic strains rather than epidemic spread of resistant isolates.

Molecular Epidemiology of Antibiotic-Resistant Escherichia coli Isolated from Hospitalized Patients with Urinary Tract Infections in Northern Palestine

Tue, 2013-03-05 20:17 — Ghaleb Adwan

Journal Title, Volume, Page:

. Polish Journal of Microbiology. 2004; 53(1): 23-26.

Year of Publication:

2004

Link:

http://www.ncbi.nlm.nih.gov/pubmed/15330263

Authors:

Adwan K,

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Abu-Hasan N

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Adwan G

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Current Affiliation:

Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine

[email protected]

Jarrar N

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Abu-Shanab B

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Al-Masri M

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Preferred Abstract (Original):

Eighty isolates of Escherichia coli were collected in Northern Palestine throughout the 1996 to 2000 period from hospitalized patients with urinary tract infections (UTIs). Resistance rates were ampicillin, 65%; co-trimoxazole, 55%; cefuroxime, 10%; cefotaxime, 7.5%; ceftazidime, 2.5%; ciprofloxacin, 12.5%; gentamicin, 6.25% and amikacin, 1.25%. No imipenem-resistant isolates were identified. To determine whether this was due to intra-hospital transmission of resistant strains, clonal structure of 10 multiple-resistant isolates was examined by genomic DNA fingerprinting by enterobacterial repetitive intergenic concensus-polymerase chain reaction (ERICPCR) and all were clonally distinct. Thus, these strains are likely resistant due to convergent acquisition of resistance determinants by genetically unrelated uropathogenic strains rather than epidemic spread of resistant isolates.

Molecular Epidemiology of Antibiotic-Resistant Escherichia coli Isolated from Hospitalized Patients with Urinary Tract Infections in Northern Palestine

Mon, 2013-03-04 13:10 — Kamel Mohammed Dauod Adwan

Journal Title, Volume, Page:

Polish Journal of Microbiology 2004, Vol. 53, No 1, 23-26

Year of Publication:

2004

Link:

http://www.ncbi.nlm.nih.gov/pubmed/15330263

Authors:

Adwan K.

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Current Affiliation:

Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine

[email protected]

Abu-Hasan N.

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Adwan G

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Jarrar N

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Abu-Shanab B

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Al-Masri M

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Preferred Abstract (Original):

Eighty isolates of Escherichia coli were collected in Northern Palestine throughout the 1996 to 2000 period from hospitalized patients with urinary tract infections (UTIs). Resistance rates were ampicillin, 65%; co-trimoxazole, 55%; cefuroxime, 10%; cefotaxime, 7.5%; ceftazidime, 2.5%; ciprofloxacin, 12.5%; gentamicin, 6.25% and amikacin, 1.25%. No imipenem-resistant isolates were identified. To determine whether this was due to intra-hospital transmission of resistant strains, clonal structure of 10 multiple-resistant isolates was examined by genomic DNA fingerprinting by enterobacterial repetitive intergenic concensus-polymerase chain reaction (ERIC-PCR) and all were clonally distinct. Thus, these strains are likely resistant due to convergent acquisition of resistance determinants by genetically unrelated uropathogenic strains rather than epidemic spread of resistant isolates.

Molecular Epidemiology of Antibiotic-Resistant Escherichia coli Isolated from Hospitalized Patients with Urinary Tract Infections in Northern Palestine

Fri, 2013-03-01 14:11 — Naser Rushdi Mahmoud Jarrar

Journal Title, Volume, Page:

Polish Journal of Microbiology 2004, Vol. 53, No 1, 23-26

Year of Publication:

2004

Authors:

K. ADWAN

Department of Biological Sciences, An-Najah National University, Nablus, P. O. Box 7, Palestine

N. ABU-HASAN

Department of Biological Sciences, An-Najah National University, Nablus, P. O. Box 7, Palestine

G. ADWAN

Department of Biological Sciences, An-Najah National University, Nablus, P. O. Box 7, Palestine

N. JARRAR

Department of Biological Sciences, An-Najah National University, Nablus, P. O. Box 7, Palestine

Current Affiliation:

Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine

[email protected]

B. ABU-SHANAB

Department of Biological Sciences, An-Najah National University, Nablus, P. O. Box 7, Palestine

M. AL-MASRI

Department of Biological Sciences, An-Najah National University, Nablus, P. O. Box 7, Palestine

Preferred Abstract (Original):

Abstract Eighty isolates of Escherichia coli were collected in Northern Palestine throughout the 1996 to 2000 period from hospitalized patients with urinary tract infections (UTIs). Resistance rates were ampicillin, 65%; co-trimoxazole, 55%; cefuroxime, 10%; cefotaxime, 7.5%; ceftazidime, 2.5%; ciprofloxacin, 12.5%; gentamicin, 6.25% and amikacin, 1.25%. No imipenem-resistant isolates were identified. To determine whether this was due to intra-hospital transmission of resistant strains, clonal structure of 10 multiple-resistant isolates was examined by genomic DNA fingerprinting by enterobacterial repetitive intergenic concensus-polymerase chain reaction (ERICPCR) and all were clonally distinct. Thus, these strains are likely resistant due to convergent acquisition of resistance determinants by genetically unrelated uropathogenic strains rather than epidemic spread of resistant isolates. Key words: E. coli, antibiotic-resistance, DNA fingerprinting, ERIC-PCR * Correspondence to: Dr. K. Adwan, Department of Biological Sciences, An-Najah N. University, P. O. Box (7)-Nablus, Palestine. Fax: 972 9 387 982; e-mail: adwank@yahoo.com

Molecular Epidemiology of Antibiotic-Resistant Escherichia Coli Isolated From Hospitalized Patients with Urinary Tract Infections in Northern Palestine

Wed, 2013-02-20 12:16 — Bassam Abu Shanab

Journal Title, Volume, Page:

Pol J Microbiol.;53(1):23-6

Year of Publication:

2004

Link:

http://www.ncbi.nlm.nih.gov/pubmed/15330263

Authors:

Abu-Shanab B

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Current Affiliation:

Department of Animal Production and Health, Faculty of Agriculture and Veterinary Medicine, An Najah National University, Nablus, Palestine

[email protected]

Adwan K

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

[email protected]

Abu-Hasan N

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Adwan G

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Jarrar N

Department of Biological Sciences, An-Najah N University, Nablus, Palestine

Al-Masri M

Preferred Abstract (Original):

Eighty isolates of Escherichia coli were collected in Northern Palestine throughout the 1996 to 2000 period from hospitalized patients with urinary tract infections (UTIs). Resistance rates were ampicillin, 65%; co-trimoxazole, 55%; cefuroxime, 10%; cefotaxime, 7.5%; ceftazidime, 2.5%; ciprofloxacin, 12.5%; gentamicin, 6.25% and amikacin, 1.25%. No imipenem-resistant isolates were identified. To determine whether this was due to intra-hospital transmission of resistant strains, clonal structure of 10 multiple-resistant isolates was examined by genomic DNA fingerprinting by enterobacterial repetitive intergenic concensus-polymerase chain reaction (ERIC-PCR) and all were clonally distinct. Thus, these strains are likely resistant due to convergent acquisition of resistance determinants by genetically unrelated uropathogenic strains rather than epidemic spread of resistant isolates.

Antibiotic Resistance of Bacterial Strains Isolated from Patients with Community-Acquired Urinary Tract Infections: An Exploratory Study in Palestine

Thu, 2013-02-14 16:37 — Professor Waleed M. Sweileh

Journal Title, Volume, Page:

Curr Clin Pharmacol. 2011, 6(4):304-7

Year of Publication:

2011

Link:

http://www.ncbi.nlm.nih.gov/pubmed/22082328

Authors:

Sweileh WM

Department of Pharmacology and Toxicology, College of Pharmacy, An-Najah National University, Nablus, Palestine

Current Affiliation:

College of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine

[email protected]

Abu-Taha AS

Department of Pharmacology and Toxicology, College of Pharmacy, An-Najah National University, Nablus, Palestine

Preferred Abstract (Original):

BACKGROUND AND OBJECTIVES:

No studies about resistance of bacteria isolated from patients with community-acquired urinary tract infections (CA-UTI) or local guidelines for antibiotic use in these infections have been published or established in the West Bank, Palestine. The objectives of this study were to determine the (1) type and frequency of isolated bacteria and (2) their resistance to commonly used antibiotics.

METHODS:

A cross sectional study on community urinary isolates was carried out in Nablus, Palestine between November 2009 and April 2010. A convenience sampling method was used for collection of specimens.

RESULTS:

A total of 375 specimens were collected from 306 (81.6%) females and 69 (18.4%) males. Three hundred and thirty nine (90.4%) of isolated uropathogens were Gram-negative bacteria, of which 243 (71.7%) were Escherichia coli. Thirty six (9.6 %) of the total isolates were Gram-positive bacteria, of which 21 (58.3%) were Staphylococcus saprophyticus. High resistance rates were recorded for E. coli against trimethoprim/sulfamethoxazole (37%), nitrofurantoin (29%), ampicillin (65%), and nalidixic acid (37%). E. coli showed low resistance to amoxicillin/clavulanic acid, ciprofloxacin, cefotaxime and ceftriaxone with rates of 12.2, 17.2, 11.1, and 11.1% respectively.

CONCLUSION:

E. coli was the most frequent bacterium in the studied sample and showed high resistance to first-line antibiotics. Our results highlight the need for developing local guidelines where elevated resistance to antibiotics should influence prescribing decisions.