The effect of water stress induced on cell membrane stability was examined in two plants, (Hordeum vulgare L) and Pearlmilletusingplant growth promotion(PGPR). Brackish water as byproduct from reverse osmosis plant (RO) after desalination process, considered as unfriendly environmentally impact and affects agriculture growth. It contains significant concentrations of dissolved salts ions such as Na+, Cl- , Ca2+, Mg2+, K1+, SO2-, and CO32- as major ions. Total dissolved salts (TDS) of these ions ranged from (5000 mg/L -10000 mg/L). Salt ions accumulation was found to be increased in shoots of barly and pearlmillet (159.09mmol, 179.73mmol) /0.114m2 of pots while TDS for decant water decreased to reach (0.101 mg/L). Electrolyte leakage assay showed that plant treated with PGPRs resulted in same values for trials treated with fresh water and less electrolyte leakage from membrane equal to 304 mg/L. The novel results of this research study that carried for the first time where PGPRs Pseudomonas putida (UW3 and UW4) had been used for improving the phytoremediation activities of two salt tolerant plants: Barley (Hordeum valgare L.) and Pearlmillet plants had showed a very clear and significant improvements of high salt uptake and thus high phytoremediation activities of these plants once they were treated with PGPRs.
No studies about resistance of bacteria isolated from patients with community-acquired urinary tract infections (CA-UTI) or local guidelines for antibiotic use in these infections have been published or established in the West Bank, Palestine. The objectives of this study were to determine the (1) type and frequency of isolated bacteria and (2) their resistance to commonly used antibiotics.
A cross sectional study on community urinary isolates was carried out in Nablus, Palestine between November 2009 and April 2010. A convenience sampling method was used for collection of specimens.
A total of 375 specimens were collected from 306 (81.6%) females and 69 (18.4%) males. Three hundred and thirty nine (90.4%) of isolated uropathogens were Gram-negative bacteria, of which 243 (71.7%) were Escherichia coli. Thirty six (9.6 %) of the total isolates were Gram-positive bacteria, of which 21 (58.3%) were Staphylococcus saprophyticus. High resistance rates were recorded for E. coli against trimethoprim/sulfamethoxazole (37%), nitrofurantoin (29%), ampicillin (65%), and nalidixic acid (37%). E. coli showed low resistance to amoxicillin/clavulanic acid, ciprofloxacin, cefotaxime and ceftriaxone with rates of 12.2, 17.2, 11.1, and 11.1% respectively.
E. coli was the most frequent bacterium in the studied sample and showed high resistance to first-line antibiotics. Our results highlight the need for developing local guidelines where elevated resistance to antibiotics should influence prescribing decisions.