Organic and aqueous solvent extracts and fractions of Micromeria nervosa (Desf.) Benth. (Labiatae) were investigated for antimicrobial activities on several microorganisms including bacteria and yeast. The different extracts differed significantly in their antimicrobial activities with the ethanolic extract being most active, followed by ethyl acetate and petroleum ether extracts. The least active extract was the aqueous. No significant difference between the different test microorganisms in their susceptibility to different extracts, with Candida albicans being the most susceptible to ethanolic extract giving 1.5 times the effect of nystatin. One of the main active ingredients of M. nervosa was isolated and identified as carvacrol. No significant difference in antimicrobial activity was found between carvacrol and its isomer thymol. The most susceptible test microorganism was Proteus vulgaris to carvacrol, and P. vulgaris and C. albicans to thymol. The least susceptible bacterium to both fractions was Pseudomonas aeruginosa. Minimum inhibitory concentration (MIC) and minimum bactericidal or fungicidal concentrations (MBC or MFC) were determined for both compounds.
The antimicrobial activities of 56 Palestinian medicinal plants against etiologic agents of acne vulgaris, mainly Propionibacterium acnes and Staphylococcus aureus was studied using disc diffusion and broth dilution methods. The results from the disc diffusion method demonstrated that these plants differ significantly in their activity against the studied microorganisms. The most active plants against all bacterial strains were Rhus coriaria, Ricinus communis, and Sarcopoterium spinosum. Test microorganisms differed significantly in relation to their susceptibility to different plant extracts used. Generally, anaerobic bacteria were more susceptible to plant extracts than aerobic bacteria. Those plants which could inhibit the growth of P. acnes, R. coriaria, R. communis, and S. spinosum had strong inhibitory effects. 43 plants could inhibit the growth of all aerobic bacteria. Based on a broth dilution method, the R. coriaria extract had the greatest antimicrobial effect against P. acnes (MIC 6 mg/ml, MBC 6 mg/ml), S. aureus (MIC 4 mg/ml, MBC 6 mg/ml), E. coli (MIC 6 mg/ml, MBC 8 mg/ml)and P. aeruginosa (4 and 6 mg/ml).Taken together, our data indicate that R. coriaria, R. communis had a strong inhibitory effect on P. acnes and most other test bacteria. Therefore, the two plants would be an interesting topic for further study and possibly for an alternative treatment for acne.
Organic and aqueous solvent extracts and fractions of Micromeria nervosa (Desf.) Benth. (Labiatae) were investigated for antimicrobial activities on several microorganisms including bacteria and yeast. The different extracts differed significantly in their antimicrobial activities with the ethanolic extract being most active, followed by ethyl acetate and petroleum ether extracts. The least active extract was the aqueous. No significant difference between the different test microorganisms in their susceptibility to different extracts, with Candida albicans being the most susceptible to ethanolic extract giving 1.5 times the effect of nystatin. One of the main active ingredients of M. nervosa was isolated and identified as carvacrol. No significant difference in antimicrobial activity was found between carvacrol and its isomer thymol. The most susceptible test microorganism was Proteus vulgaris to carvacrol, and P. vulgaris and C. albicans to thymol. The least susceptible bacterium to both fractions was Pseudomonas aeruginosa. Minimum inhibitory concentration (MIC) and minimum bactericidal or fungicidal concentrations (MBC or MFC) were determined for both compounds.
A series of curcumin derivatives containing heterocyclic moiety have been synthesized and evaluated for their antibacterial activities. The chemical structures of the synthesized compounds were verified on the basis of spectral data and elemental analyses. Investigation of antimicrobial activity of the derivatives demonstrated the ability to inhibit Gram-positive microorganisms with zone of inhibition ranging from 14-18 mm, MIC ranging between 0.0625 and 0.25 mg/mL. Among all tested derivatives, diazepine 4 exhibited remarkable potency against Gram-positive bacteria S. aureus. An extensive study is underway to optimize the effectiveness of diazepine type of compounds and to determine their mode of action.
A series of curcumin derivatives containing heterocyclic moiety have been synthesized and evaluated for their antibacterial activities. The chemical structures of the synthesized compounds were verified on the basis of spectral data and elemental analyses. Investigation of antimicrobial activity of the derivatives demonstrated the ability to inhibit Gram-positive microorganisms with zone of inhibition ranging from 14-18 mm, MIC ranging between 0.0625 and 0.25 mg/mL. Among all tested derivatives, diazepine 4 exhibited remarkable potency against Gram-positive bacteria S. aureus. An extensive study is underway to optimize the effectiveness of diazepine type of compounds and to determine their mode of action.