A method for the spectrophotometric determination of cobalt(II) is presented, with a comparison of the binary complexes formed by cobalt(II) with di-2-pyridyl ketone nicotinoylhydrazone (DPKNH), di-2-pyridyl ketone 2-thiophenoylhydrazone (DPKTH) and di-2-pyridyl ketone benzoylhydrazone (DPKBH) in 50% (v/v) ethanolic solution. Cobalt(II) forms 1:2 complexes with the three reagents. Maximum absorbance is at 372 nm for Co(II)-DPKNH, at 389 nm for Co(II)-DPKTH and at 370 nm for Co(II)-DPKBH. Ranges of linearity, effects of pH and excess reagent, sensitivity, stability of the complexes and tolerance limits of ions are reported. It was concluded that the system with DPKTH is the best, followed by DPKNH and then DPKBH. The method was applied to the determination of cobalt in different alloys.
A method for the spectrophotometric determination of cobalt(II) is presented, with a comparison of the binary complexes formed by cobalt(II) with di-2-pyridyl ketone nicotinoylhydrazone (DPKNH), di-2-pyridyl ketone 2-thiophenoylhydrazone (DPKTH) and di-2-pyridyl ketone benzoylhydrazone (DPKBH) in 50% (v/v) ethanolic solution. Cobalt(II) forms 1:2 complexes with the three reagents. Maximum absorbance is at 372 nm for Co(II)-DPKNH, at 389 nm for Co(II)-DPKTH and at 370 nm for Co(II)-DPKBH. Ranges of linearity, effects of pH and excess reagent, sensitivity, stability of the complexes and tolerance limits of ions are reported. It was concluded that the system with DPKTH is the best, followed by DPKNH and then DPKBH. The method was applied to the determination of cobalt in different alloys.
The spectral and differential pulse polarographic (DPP) behaviour of di-2-pyridyl ketone 2-thienoylhydrazone (DPKTH) has been investigated in 30% (v/v) ethanol-buffer mixtures over a wide range of pH (2.0-12.0). The spectral bands located at 325, 276, 230 and 208 nm of the DPKTH in ethanol are assigned to the possible electronic transitions of the molecule. The spectral data at various pH values indicates that the molecule characterized by the keto = enol tautomerization and the pKa value for the enolic form of the compound is determined in aqueous ethanol and was found to be 10.6. The effect of various operational parameters on the reduction current and the mechanism of the electrode reaction of DPKTH at the DME are discussed. The main reduction peaks are attributed to the reduction of C=N centre of both the keto and enol forms. This behaviour is compared with the DPP behaviour of the other related acid hydrazone compounds. The applicability of DPP technique for the trace determination of DPKTH was tested under the optimum experimental conditions and the detection limit is found to be 0.09 μm.
A method for spectrophotometric determination of nitrite and nitrate is described. This method is based on the reduction of phosphomolybdic acid to phosphomolybdenum blue complex by sodium sulfide. The obtained phosphomolybdenum blue complex is oxidized by the addition of nitrite and this causes a reduction in intensity of the blue color. The absolute decrease in the absorbance of the blue color or the rate of its decrease is found to be directly proportional to the amount of nitrite added. The absorbance of the phosphomolybdenum blue complex is monitored spectrophotometrically at 814 nm and related to the concentration of nitrite present. The effect of different factors such as acidity, stability of the complex, time, temperature, phosphate concentration, molybdenum concentration, sodium sulfide concentration and the tolerance amount of other ions have been reported. Maximum absorbance is at 814 nm. The range of linearity using the conventional method is 0.5–2.0 ppm with molar absorptivity of 1.1×104 l mol−1 cm−1. and a relative standard deviation of 2.6% for five measurements. The range of linearity using the reaction rate method is 0.2–3.6 ppm with a relative standard deviation of 2.4% for five measurements. The method is applied for determination of nitrite and nitrate in water, meat products and vegetables.
A method for the spectrophotometric determination of cobalt(II) is presented, with a comparison of the binary complexes formed by cobalt(II) with di-2-pyridyl ketone nicotinoylhydrazone (DPKNH), di-2-pyridyl ketone 2-thiophenoylhydrazone (DPKTH) and di-2-pyridyl ketone benzoylhydrazone (DPKBH) in 50% (v/v) ethanolic solution. Cobalt(II) forms 1:2 complexes with the three reagents. Maximum absorbance is at 372 nm for Co(II)-DPKNH, at 389 nm for Co(II)-DPKTH and at 370 nm for Co(II)-DPKBH. Ranges of linearity, effects of pH and excess reagent, sensitivity, stability of the complexes and tolerance limits of ions are reported. It was concluded that the system with DPKTH is the best, followed by DPKNH and then DPKBH. The method was applied to the determination of cobalt in different alloys.
A method for spectrophotometric determination of nitrite and nitrate is described. This method is based on the reduction of phosphomolybdic acid to phosphomolybdenum blue complex by sodium sulfide. The obtained phosphomolybdenum blue complex is oxidized by the addition of nitrite and this causes a reduction in intensity of the blue color. The absolute decrease in the absorbance of the blue color or the rate of its decrease is found to be directly proportional to the amount of nitrite added. The absorbance of the phosphomolybdenum blue complex is monitored spectrophotometrically at 814 nm and related to the concentration of nitrite present. The effect of different factors such as acidity, stability of the complex, time, temperature, phosphate concentration, molybdenum concentration, sodium sulfide concentration and the tolerance amount of other ions have been reported. Maximum absorbance is at 814 nm. The range of linearity using the conventional method is 0.5–2.0 ppm with molar absorptivity of 1.1×104 l mol−1 cm−1. and a relative standard deviation of 2.6% for five measurements. The range of linearity using the reaction rate method is 0.2–3.6 ppm with a relative standard deviation of 2.4% for five measurements. The method is applied for determination of nitrite and nitrate in water, meat products and vegetables.
A method for the spectrophotometric determination of cobalt(II) is presented, with a comparison of the binary complexes formed by cobalt(II) with di-2-pyridyl ketone nicotinoylhydrazone (DPKNH), di-2-pyridyl ketone 2-thiophenoylhydrazone (DPKTH) and di-2-pyridyl ketone benzoylhydrazone (DPKBH) in 50% (v/v) ethanolic solution. Cobalt(II) forms 1:2 complexes with the three reagents. Maximum absorbance is at 372 nm for Co(II)-DPKNH, at 389 nm for Co(II)-DPKTH and at 370 nm for Co(II)-DPKBH. Ranges of linearity, effects of pH and excess reagent, sensitivity, stability of the complexes and tolerance limits of ions are reported. It was concluded that the system with DPKTH is the best, followed by DPKNH and then DPKBH. The method was applied to the determination of cobalt in different alloys.
Two spectrophotometric methods are described for spectrophotometric determination of tiopronin in pharmaceuticals. They are based on the oxidation-reduction reaction between tiopronin and iron(III)-ferrozine complex [system I] or iron(III)-di-2-pyridyl ketone-2-thiophenoyl-hydrazone complex [system II]. The produced colored iron(II)-Ferrozine or iron(II)-di-2-pyridyl ketone-2-thiophenoyl-hydrazone complex absorbs at 562 nm and 656 nm, respectively. The effect of different factors such as; pH, reagent concentration, times, temperature and the tolerance amount of the common excipients have been reported. Applying the optimum working conditions, tiopronin can be determined over the range 0.2-8.6 and 0.5-17.0 ppm when using system I and II, respectively. The two methods offer high selectivity, sensitivity and accuracy with RSD less than 1.5% for five measurements. The proposed methods were applied successfully for the determination of tiopronin in Captimer tablets.
Two methods are described for quantitative determination
of nizatidine. The first is a cathodic stripping voltammetric method which is
based on the accumulation of the compound at the hanging mercury drop
electrode. The adsorptive stripping response was evaluated with respect of
accumulation time, potential, concentration, pH and other variables. A linear
calibration graph was obtained over the range 3.0×10−8–1.0×10−6 M
with a detection limit 3.0×10−8 M after a 20s accumulation time at
−0.2 V accumulation potential. On the other hand, it was found that the
detection limit could be lowered to 1.0×10−8 M after 180s
accumulation time at −0.2 V accumulation potential. The relative standard
deviation was in the range 1.2−2.0% for six measurements. The tolerance amounts
of the common excipients have also been reported.
The second is a spectrophotometric method which is based
on the formation and extraction of the ion-pair complex formed between
nizatidine and either bromocresol green or bromothymol blue. The extracted
colored ion-pair complexes absorb at 416 nm. The effect of different factors
such as: type of organic solvent, pH, reagent concentration, number of
extraction times, shaking time, temperature and the tolerance amount of the
common excipients have been reported. The calibration graph was linear in the
range 6.0×10−7–1.8×10−5 M with a detection limit of 6.0×10−7 M
and molar absorptivity of 2.1×104 lċmol−1ċcm−1
when using bromocresol green, while the calibration graph was linear in the
range 3.0×10−7–1.1×10−5 M with a detection limit of
3.0×10−7 M and molar absorptivity of 3.2×104 lċmol−1ċcm−1
when using bromothymol blue. The spectrophotometric methods offer alternative
methods with reasonable sensitivity, selectivity and accuracy with relative
standard deviation in the range 2.1−6.0% and 1.2−4.7% (for six measurements)
when using bromothymol blue and bromocresol green, respectively. The proposed
two methods were applied for the determination of nizatidine in commercially
available dosage forms. A comparison between the voltammetric and the
extraction-spectrophotometric methods was also reported.