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Spectrophotometric Determination of ‎Cysteine and N-acetylcysteine in ‎Pharmaceutical Preparations

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Thu, 2014-03-27 12:29 — Maher An-Natsheh
Journal Title, Volume, Page: 
Microchimica Acta, Volume 129, Issue 1-2, pp 91-95
Year of Publication: 
1998
Link: 
http://link.springer.com/article/10.1007%2FBF01246854
Authors: 
Maher Abu Eid
Chemistry Department , An-Najah National University , Nablus, Palestine
Current Affiliation: 
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
[email protected]
Preferred Abstract (Original): 

An accurate fast spectrophotometric method for the determination of cysteine andN-acetyl-cysteine is presented, based on the oxidation of these amino acids by ferric ions in the presence of ferrozine, whereby a violet-coloured complex is formed which absorbs at 562 nm. The method was satisfactory for the determination of cysteine andN-acetylcysteine in samples within the range 0.02–6.00 μgml−1. Effects of time, acidity, ferric ions, ferrozine, sodium perchlorate concentrations and the tolerance limit for other amino acids have been reported. The method was applied to the determination of cysteine in amino acids mixtures andN-acetylcysteine in pharmaceutical preparations.