epidemiology

adwank's picture

Molecular Characterization of Methicillin-Resistant Staphylococcus Aureus Isolates in Three Different Arab World Countries

Journal Title, Volume, Page: 
Eur J Microbiol Immunol (Bp). Sep 2013; 3(3): 183–187. Published online Sep 23, 2013. doi:10.1556/EuJMI.3.2013.3.5
Year of Publication: 
2003
Authors: 
Israr Sabri
Master Program in Clinical Laboratory Science (MCLS), Birzeit University, Birzeit, Palestine
Kamel M. Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Tamer A Essawi
Master Program in Clinical Laboratory Science (MCLS), Birzeit University, Birzeit, Palestine
Mohammad A Farraj
Master Program in Clinical Laboratory Science (MCLS), Birzeit University, Birzeit, Palestine
Preferred Abstract (Original): 

Molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) isolates in three different Arab world countries (West Bank of Palestine, Jordan, and Iraq) was the aim of the study presented here. This is done on the basis of spa sequencing and staphylococcal cassette chromosome mec (SCCmec) typing. The majority (92%) of the spa-tested isolates belonged to spa type t932 and possessed the (SCCmec) type III. These data suggest that MRSA clone, which harbors the spa type t932 and (SCCmec) type III, had been transferred throughout the three studied countries.

nsabuhasan's picture

Typing of Methicillin-Resistant Staphylococcus Aureus By Ribosome Spacer And Arbitrarily Primed Polymerase Chain Reaction

Journal Title, Volume, Page: 
Turkish Journal of Medical Sciences, 34 (2004) 5-9
Year of Publication: 
2004
Authors: 
Tamer Essaw
Master Program in Clinical Laboratory Sciences, Birzeit University, Palestine
Kamel M. Adwan
Nael Abu-Hassan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Galeb Adwan
Department of Biological Sciences, An-Najah N. University, Nablus, Palestine
Ahmed Saleh
Department of Biological Sciences, An-Najah National University, Nablus, P. O. Box 7, Palestine
Preferred Abstract (Original): 

Thirty-five methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates were collected from 3 hospitals in the northern and southern parts of Palestine between February and May 1998. These isolates were typed by ribosome spacer PCR (RSPCR)and arbitrarily primed PCR (AP-PCR). RS-PCR generated 9 different genotypes. The use of APÐPCR provided a high resolution typing method and allowed us to define 11 different clusters. Three major clusters, however, based on the combination of both typing methods, spread throughout the neonatal and intensive care units of Rafidya Hospital during the entire period.

awni2003's picture

Molecular Characterization of Escherichia Coli Isolates from Patients with Urinary Tract Infections in Palestine

Journal Title, Volume, Page: 
J Med Microbiol. 2014 Feb;63(Pt 2):229-34. doi: 10.1099/jmm.0.067140-0. Epub 2013 Nov 15
Year of Publication: 
2013
Authors: 
Kamel Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Naser Jarrar
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Naser Jarrar
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Awni Abu-Hijleh
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Ghaleb Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Elena Awwad
Central Veterinary Laboratory, Directorate of Veterinary Services and Animal Health, Ministry of Agriculture, Palestine
Preferred Abstract (Original): 

Antibiotic resistance of Escherichia coli isolated from urinary tract infections (UTIs) is increasing worldwide. A total of 41 E. coli isolates were obtained from urine samples from hospitalized patients with a UTI in three hospitals in the northern districts of the West Bank, Palestine during March and June 2011. Resistance rates were: erythromycin (95 %), trimethoprim–sulfamethoxazole (59 %), ciprofloxacin (56 %), gentamicin (27 %), imipenem (22 %), amoxicillin (93 %), amoxicillin–clavulanic acid (32 %), ceftazidime (66 %) and cefotaxime (71 %). No meropenem-resistant isolates were identified in this study. Among the isolates, phylogenetic group B2 was observed in 13 isolates, D in 12 isolates, A in 11 isolates and B1 in five isolates. Thirty-five of the isolates were positive for an extended-spectrum β-lactamase phenotype. Among these isolates, the blaCTX-M gene was detected in 25, and eight harboured the blaTEM gene. None of the isolates contained the blaSHV gene. Transformation experiments indicated that some of the β-lactamase genes (i.e. blaCTX-M and blaTEM) with co-resistance to erythromycin and gentamicin were plasmid encoded and transmissible. Apart from this, enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) revealed that the 41 isolates were genetically diverse and comprised a heterogeneous population with 11 ERIC-PCR profiles at a 60 % similarity level.
The GenBank/EMBL/DDBJ accession numbers for three blaCTX-M gene sequences and one blaTEM gene sequence of Escherichia coli are KF696718, KF696719, KF696720 and KF696717, respectively.

Abbreviations:
ERIC
enterobacterial repetitive intergenic consensus
ESBL
extended-spectrum β-lactamase
UPGMA
unweighted pair group method for arithmetic averages
UTI
urinary tract infection

adwank's picture

Typing of Methicillin-Resistant Staphylococcus Aureus by Ribosome Spacer and Arbitrarity Primed Polymerase Chain Reaction

Journal Title, Volume, Page: 
Turkiye Klinikleri Journal of Medical Sciences , 01/2004; 34
Year of Publication: 
2004
Authors: 
Tamer Essawi
Master Program in Clinical Laboratory Sciences, Birzeit University, Palestine
K Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
N. Abu-Hassan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
G.Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
A. Saleh
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Preferred Abstract (Original): 

Thirty-five methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates were collected from 3 hospitals in the northern and southern parts of Palestine between February and May 1998. These isolates were typed by ribosome spacer PCR (RSPCR) and arbitrarily primed PCR (AP-PCR). RS-PCR generated 9 different genotypes. The use of APÐPCR provided a high resolution typing method and allowed us to define 11 different clusters. Three major clusters, however, based on the combination of both typing methods, spread throughout the neonatal and intensive care units of Rafidya Hospital during the entire period.

adwank's picture

Molecular Characterization of Escherichia Coli Isolates from Patients with Urinary Tract Infections in Palestine

Journal Title, Volume, Page: 
J Med Microbiol. 2014 Feb;63(Pt 2):229-34. doi: 10.1099/jmm.0.067140-0. Epub 2013 Nov 15
Year of Publication: 
2013
Authors: 
Kamel Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Naser Jarrar
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Awni Abu-Hijleh
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Ghaleb Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Elena Awwad
Central Veterinary Laboratory
Preferred Abstract (Original): 

Antibiotic resistance of Escherichia coli isolated from urinary tract infections (UTIs) is increasing worldwide. A total of 41 E. coli isolates were obtained from urine samples from hospitalized patients with a UTI in three hospitals in the northern districts of the West Bank, Palestine during March and June 2011. Resistance rates were: erythromycin (95 %), trimethoprim-sulfamethoxazole (59 %), ciprofloxacin (56 %), gentamicin (27 %), imipenem (22 %), amoxicillin (93 %), amoxicillin-clavulanic acid (32 %), ceftazidime (66 %) and cefotaxime (71 %). No meropenem-resistant isolates were identified in this study. Among the isolates, phylogenetic group B2 was observed in 13 isolates, D in 12 isolates, A in 11 isolates and B1 in five isolates. Thirty-five of the isolates were positive for an extended-spectrum β-lactamase phenotype. Among these isolates, the blaCTX-M gene was detected in 25, and eight harboured the blaTEM gene. None of the isolates contained the blaSHV gene. Transformation experiments indicated that some of the β-lactamase genes (i.e. blaCTX-M and blaTEM) with co-resistance to erythromycin and gentamicin were plasmid encoded and transmissible. Apart from this, enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) revealed that the 41 isolates were genetically diverse and comprised a heterogeneous population with 11 ERIC-PCR profiles at a 60 % similarity level.

MSShtayeh's picture

Keratinophilic Fungi on the Hair of Goats from the West Bank of Jordan

Journal Title, Volume, Page: 
Mycopathologia November 1988, Volume 104, Issue 2, pp 103-108
Year of Publication: 
1988
Authors: 
M. S. Ali-Shtayeh
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus. Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus. Palestine
H. M. Arda
Arda Dermatology Clinic, Nablus, Palestine
M. Hassouna
Department of Veterinary, Nablus, Palestine
S. F. Shaheen
Department of Public Health, Nablus, Palestine
Preferred Abstract (Original): 

The mycoflora of the hair in 178 goats from the West Bank of Jordan was analysed and the frequency of occurrence and the relative importance value for the different keratinophilic fungi found were calculated. One hundred and seven species which belong to 38 genera were isolated. Thirty six of these species were either well recognised agents of mycoses (Trichophyton mentagrophytes, T. verrucosum, and M. nanum), or have been frequently isolated from human and animal lesions (Arthroderma spp., Acremonium kiliense, Alternaria alternata, Aspergillus flavus, Cladosporium carionii, and several other species). These potentially pathogenic fungal species comprised 66.9% of all keratinophilic fungi found on the hair of goats. The role of this animal as a reservoir for dermatophytes and other potentially pathogenic fungi is discussed.

MSShtayeh's picture

Keratinophilic Fungi on the Hair of Cows, Donkeys, Rabbits, Cats, and Dogs from the West Bank of Jordan

Journal Title, Volume, Page: 
Mycopathologia November 1988, Volume 104, Issue 2, pp 109-121
Year of Publication: 
1988
Authors: 
M. S. Ali-Shtayeh
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus. Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus. Palestine
H. M. Arda
Arda Dermatology Clinic, Nablus
M. Hassouna
Department of Veterinary, Nablus
S. F. Shaheen
Department of Public Health, Nablus
Preferred Abstract (Original): 

The mycoflora of hair of 111 cows, donkeys, rabbits, cats, and dogs were analysed and the frequency of occurrence and the relative importance value of the different fungal species isolated were calculated. Total numbers of species 56, 45, 48, 23, and 11 were recovered from cows, donkeys, rabbits, cats, and dogs respectively. The majority of the keratinophilic fungi isolated were either well-known mycotic agents or have been recovered from various animal and human lesions. These comprised 87.8%, 66.7%, 61.4%, 59.3%, and 55.2% of the hair mycoflora in cats, dogs, rabbis, cows, and donkeys respectively. Seven species of dermatophytes were isolated: Trichophyton mentagrophytes, T. verrucosum, Microsporum gypseum, M. nanum, T. ajelloi, and M. canis. Cats harboured the largest number of dermatophytes (5 species), followed by cows (4 spp), rabbits (3 spp), and donkeys and dogs (1 sp). The role of these animals in the persistence and transmission of pathogenic fungi is discussed.

miamicg's picture

Daily measures of microbes and human health at a non-point source marine beach.

Journal Title, Volume, Page: 
Journal of Water and Health, 9(3): 443-457
Year of Publication: 
2011
Authors: 
Amir M. Abdelzaher
Mary E. Wright
Cristina Ortega
A. Rasem Hasan
NSF NIEHS Oceans and Human Health Center, University of Miami, 4600 Rickenbacker Causeway Miami, FL 33149, USA
Current Affiliation: 
Department of Civil Engineering, An-Najah National University, P.O. Box 7, Nablus, Palestine.
Tomoyoki Shibata
Helena M. Solo-Gabriele
Jonathan Kish
Kelly Withum
Guoqing He
Samir M. Elmir
J. Alfredo Bonilla
Tonya D. Bonilla
Carol J. Palmer
Troy M. Scott
Christopher D. Sinigalliano
Maribeth L. Gidley
David Wanless
Lisa R. W. Plano
Anna C. Garza
Xiaofang Zhu
Jay M. Fleisher
Lora E. Fleming
Lukasik J
Harwood VJ
Gidley ML
Stewart JR
Dickerson JW
Yampara-Iquise H
Carson C
Preferred Abstract (Original): 
Studies evaluating the relationship between microbes and human health at non-point source beaches are necessary for establishing criteria which would protect public health while minimizing economic burdens. The objective of this study was to evaluate water quality and daily cumulative health effects (gastrointestinal, skin, and respiratory illnesses) for bathers at a non-point source subtropical marine recreational beach in order to better understand the inter-relationships between these factors and hence improve monitoring and pollution prevention techniques. Daily composite samples were collected, during the Oceans and Human Health Beach Exposure Assessment and Characterization Health Epidemiologic Study conducted in Miami (Florida, USA) at a non-point source beach, and analyzed for several pathogens, microbial source tracking markers, indicator microbes, and environmental parameters. Analysis demonstrated that rainfall and tide were more influential, when compared to other environmental factors and source tracking markers, in determining the presence of both indicator microbes and pathogens. Antecedent rainfall and F+ coliphage detection in water should be further assessed to confirm their possible association with skin and gastrointestinal (GI) illness outcomes, respectively. The results of this research illustrate the potential complexity of beach systems characterized by non-point sources, and how more novel and comprehensive approaches are needed to assess beach water quality for the purpose of protecting bather health.
MSShtayeh's picture

Prevalence of Tinea Capitis As Well As of Asymptomatic Carriers In School Children In Nablus Area (Palestine)

Journal Title, Volume, Page: 
mycoses 45, 188–194 (2002)
Year of Publication: 
2002
Authors: 
M. S. Ali-Shtayeh
Department of Biological Sciences, An-Najah National University, Nablus, P. O. Box 7, Palestine
Current Affiliation: 
Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine
A-A. M. Salameh
Department of Biological Sciences, An-Najah University, Nablus, Palestine
S. I. Abu-Ghdeib
Department of Biological Sciences, An-Najah University, Nablus, Palestine
Rana M. Jamous
Department of Biological Sciences, An-Najah University, Nablus, Palestine
H. Khraim
Private Clinic, Nablus, Palestine
Preferred Abstract (Original): 

A study of tinea capitis was carried out during October 1998, involving 8531 school children aged 6–14 years (4718 males and 3813 females), attending 12 primary schools located in urban, rural, and refugee camp communities in the Nablus district in the Palestinian Authority. A total of 1389 of the school children aged 6–12 years (724 males and 665 females) were also surveyed on three occasions at 2–3 month intervals, over a 9-month period (October 1998–May 1999) using the hair brush technique, for prevalence of asymptomatic tinea capitis carriage. Twenty-three (0.27%) mycologically proven cases of tinea capitis were detected

adwang's picture

Typing of Methicillin-Resistant Staphylococcus Aureus By Ribosome Spacer And Arbitrarily Primed Polymerase Chain Reaction

Journal Title, Volume, Page: 
Turkish Journal of Medical Sciences, 34 (2004) 5-9
Year of Publication: 
2004
Authors: 
Tamer Essaw
Master Program in Clinical Laboratory Sciences, Birzeit University, Palestine
Kamel M. Adwan
Department of Biological Sciences, An-Najah National University, Nablus, P. O. Box 7, Palestine
Nael Abu-Hassan
Department of Biological Sciences, An-Najah National University, Nablus, P. O. Box 7, Palestine
Galeb Adwan
Department of Biological Sciences, An-Najah N. University, Nablus, Palestine
Current Affiliation: 
Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine
Ahmed Saleh
Department of Biological Sciences, An-Najah National University, Nablus, P. O. Box 7, Palestine
Preferred Abstract (Original): 

Thirty-five methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates were collected from 3 hospitals in the northern and southern parts of Palestine between February and May 1998. These isolates were typed by ribosome spacer PCR (RSPCR)and arbitrarily primed PCR (AP-PCR). RS-PCR generated 9 different genotypes. The use of APÐPCR provided a high resolution typing method and allowed us to define 11 different clusters. Three major clusters, however, based on the combination of both typing methods, spread throughout the neonatal and intensive care units of Rafidya Hospital during the entire period.

Syndicate content