Typing of Methicillin-Resistant Staphylococcus Aureus By Ribosome Spacer And Arbitrarily Primed Polymerase Chain Reaction

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Journal Title, Volume, Page: 
Turkish Journal of Medical Sciences, 34 (2004) 5-9
Year of Publication: 
2004
Authors: 
Tamer Essaw
Master Program in Clinical Laboratory Sciences, Birzeit University, Palestine
Kamel M. Adwan
Nael Abu-Hassan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Galeb Adwan
Department of Biological Sciences, An-Najah N. University, Nablus, Palestine
Ahmed Saleh
Department of Biological Sciences, An-Najah National University, Nablus, P. O. Box 7, Palestine
Preferred Abstract (Original): 

Thirty-five methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates were collected from 3 hospitals in the northern and southern parts of Palestine between February and May 1998. These isolates were typed by ribosome spacer PCR (RSPCR)and arbitrarily primed PCR (AP-PCR). RS-PCR generated 9 different genotypes. The use of APÐPCR provided a high resolution typing method and allowed us to define 11 different clusters. Three major clusters, however, based on the combination of both typing methods, spread throughout the neonatal and intensive care units of Rafidya Hospital during the entire period.

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