SEs

adwank's picture

Enterotoxigenic Staphylococcus Aureus in Raw Milk in the North of Palestine

Journal Title, Volume, Page: 
Turkish Journal of Biology. 01/2005; 29:229-232
Year of Publication: 
2005
Authors: 
Ghaleb Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Bassam Abu-Shanab
Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, An-Najah National University, Nablus, Palestine
Kamel Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Preferred Abstract (Original): 

Enterotoxin genes (sea-see) in Staphylococcus aureus isolates recovered from milk of clinically healthy sheep and cows in the north of Palestine were determined using a polymerase chain reaction (PCR). Thirty-seven (37%) out of 100 S. aureus isolates were toxin gene positive. Four strains (10.8%) were sea-positive, 20 (54.1%) were seb-positive, 4 (10.8%) were sec-positive, 6 (16.2%) were sed-positive and 3 (8.1%) were see-positive. None of these enterotoxigenic isolates carried more than one toxin gene. This study indicates that the presence of enterotoxigenic S. aureus in raw milk can contribute to the sources of staphylococcal food poisoning in Palestine.

adwank's picture

Enterotoxigenecity of S. Aureus Isolates Recovered From Chronic Urogenital Tract Infection in North of Palestine

Journal Title, Volume, Page: 
Pak J Med Sci, April - June 2008 (Part-I), Vol. 24 No. 2, p 246-250
Year of Publication: 
2008
Authors: 
Ghaleb Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Bassam Abu-Shanab
Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, An-Najah National University, Nablus, Palestine
Kamel Adwan
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Marwan Odeh
Al-Hikma Medical laboratories, Toulkarem, Palestine
Preferred Abstract (Original): 

Objectives: Staphylococcus aureus is an important pathogen associated with diseases in a variety of hosts including humans. It produces several toxins and virulence factors that contribute to its pathogenic potential such as staphylococcal enterotoxins (SEs). This study was conducted to determine enterotoxigenicity of S. aureus associated with chronic urogenital tract infection by detectingenterotoxin genes.
Setting: This study was done in The Microbiology laboratory, An-Najah N. University, Palestine.
Methodology: A total of 90 S. aureus isolates recovered from clinical samples from patients suffering from chronic urogenital tract infection in the North of Palestine were used to detect the presence of staphylococcal enterotoxin genes sea, seb, sec, sed and see by polymerase chain reaction (PCR) assay.
Results: Out of 90 S. aureus isolates tested, it was found that 57 (63.3%) of these isolates harboured one or more enterotoxin genes. Up to 78.9% of the enterotoxigenic isolates possessed one SE gene. The majority of these enterotoxigenic strains (61.4%) isolated from both semen and urine samples harbored sec gene either alone or in combination with other genes. Also the prevalence of genes in combination was significantly more common in S. aureus isolates derived from urine 9/33 (27.3%), as compared to those derived from semen 3/24 (12.5%).
Conclusions: The role of enterotoxin genes in the pathogenesis of urogenital tract infection is still unknown. However, it is evident that urogenital infection can be caused by S. aureus strains which lack these genes. Other newly detected genes may play a role in pathogenesis.

bassamas's picture

Toxigenicity of Staphylococcus Aureus Isolates from Northern Palestine

Journal Title, Volume, Page: 
Emirates Medical Journal (2006); 24(2):127-129
Year of Publication: 
2006
Authors: 
Bassam Ali Abu-Shanab
Faculty of Veterinary Medicine, An Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Animal Production and Health, Faculty of Agriculture and Veterinary Medicine, An Najah National University, Nablus, Palestine
Ghaleb Mohammad Adwan
Department of Biology and Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus, Palestine
Kamel Mohammad Adwan
Department Of Biology And Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus, Palestine
Naser Rushdi Jarrar
Department Of Biology And Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus, Palestine
Preferred Abstract (Original): 

A total of 68 Staphylococcus aureus strains isolated from different human clinical samples in the North of Palestine were examined to detect staphylococcal enterotoxin (SE) genes A (sea), B (seb), C (sec), D (sed) and (see). Of the total isolates examined, 41.2% (28/68) were enterotoxigenic S. aureus. Twelve strains (42.9%) of enterotoxigenic S. aureus harbored seagene, ten strains (35.7%) were carried see- gene, six strains (21.4%) were positive for sec-gene. None of these enterotoxigenic S. aureus isolates harbored more than one of toxin genes. The presence of these toxin genes and other genes not be detected here might play a role in process of pathogenesis of S. aureus disease other than food poisoning but this cannot be substantiated by the results of the present study

adwank's picture

Toxigenicity of Staphylococcus Aureus Isolates From Northern Palestine

Journal Title, Volume, Page: 
Emirates Medical Journal Volume 24, Issue 2, August 2006, Pages 127-129
Year of Publication: 
2006
Authors: 
Adwan, G.M
Department of Biology and Biotechnology, An-Najah N Univesity, PO Box 7, Nablus, Palestine
Abu-Shanab, B.A.
Faculty of Veterinary Medicine, An-Najah N University, Nablus, Palestine
Adwan, K.M.
Department of Biology and Biotechnology, An-Najah N Univesity, PO Box 7, Nablus, Palestine
Current Affiliation: 
Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine
Jarrar, N.R.
Department of Biology and Biotechnology, An-Najah N Univesity, PO Box 7, Nablus, Palestine
Preferred Abstract (Original): 
Abstract - A total of 68 Staphylococcus aureus strains isolated from different human clinical samples in the North of Palestine were examined to detect staphylococcal enterotoxin (SE) genes A (sea), B (seb), C (sec), D (sed) and (see). Of the total isolates examined, 41.2% (28/68) were enterotoxigenic S. aureus. Twelve strains (42.9%) of enterotoxigenic S. aureus harbored sea- gene, ten strains (35.7%) were carried see- gene, six strains (21.4%) were positive for sec-gene. None of these enterotoxigenic S. aureus isolates harbored more than one of toxin genes. The presence of these toxin genes and other genes not be detected here might play a role in process of pathogenesis of S. aureus disease other than food poisoning but this cannot be substanti- ated by the results of the present study. Keywords: Enterotoxigenic S. aureus, staphylococcal enterotoxins, SEs, Palestine.
Naser Jarrar's picture

Toxigenicity of Staphylococcus aureus isolates from Northern Palestine

Journal Title, Volume, Page: 
Emirates Medical Journal (2006); 24(2):
Year of Publication: 
2006
Authors: 
Ghaleb Mohammad Adwan
Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine
Bassam Ali Abu-Shanab
Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine
Kamel Mohammad Adwan
Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine
Naser Rushdi Jarrar
Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine
Preferred Abstract (Original): 
Abstract - A total of 68 Staphylococcus aureus strains isolated from different human clinical samples in the North of Palestine were examined to detect staphylococcal enterotoxin (SE) genes A (sea), B (seb), C (sec), D (sed) and (see). Of the total isolates examined, 41.2% (28/68) were enterotoxigenic S. aureus. Twelve strains (42.9%) of enterotoxigenic S. aureus harbored seagene, ten strains (35.7%) were carried see- gene, six strains (21.4%) were positive for sec-gene. None of these enterotoxigenic S. aureus isolates harbored more than one of toxin genes. The presence of these toxin genes and other genes not be detected here might play a role in process of pathogenesis of S. aureus disease other than food poisoning but this cannot be substantiated by the results of the present study. Keywords: Enterotoxigenic S. aureus, staphylococcal enterotoxins, SEs, Palestine.
bassamas's picture

Enterotoxigenecity of S. Aureusisolates Recovered from Chronic Urogenital Tract Infection in North Palestine

Journal Title, Volume, Page: 
Pak J Med Sci, (Part-I), Vol.24, No. 2, 246-250
Year of Publication: 
2008
Authors: 
Bassam Abu-Shanab
Faculty of Veterinary Medicine, An-Najah N. University, Nablus, PALESTINE
Current Affiliation: 
Department of Animal Production and Health, Faculty of Agriculture and Veterinary Medicine, An Najah National University, Nablus, Palestine
Ghaleb Adwan
Department of Biology and Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus
Kamel Adwan
Department of Biology and Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus
Marwan Odeh
Al-Hikma Medical laboratories, Toulkarem, Palestine
Preferred Abstract (Original): 
Objectives: Staphylococcus aureus is an important pathogen associated with diseases in a variety of hosts including humans. It produces several toxins and virulence factors that contribute to its pathogenic potential such as staphylococcal enterotoxins (SEs). This study was conducted to determine enterotoxigenicity of S. aureus associated with chronic urogenital tract infection by detecting enterotoxin genes. Setting: This study was done in The Microbiology laboratory, An-Najah N. University, Palestine. Methodology: A total of 90 S. aureus isolates recovered from clinical samples from patients suffering from chronic urogenital tract infection in the North of Palestine were used to detect the presence of staphylococcal enterotoxin genes sea , seb , sec, sed and see by polymerase chain reaction (PCR) assay. Results: Out of 90 S. aureus isolates tested, it was found that 57 (63.3%) of these isolates harboured one or more enterotoxin genes. Up to 78.9% of the enterotoxigenic isolates possessed one SE gene. The majority of these enterotoxigenic strains (61.4%) isolated from both semen and urine samples harbored sec gene either alone or in combination with other genes. Also the prevalence of genes in combination was significantly more common in S. aureus isolates derived from urine 9/33 (27.3%), as compared to those derived from semen 3/24 (12.5%). Conclusions: The role of enterotoxin genes in the pathogenesis of urogenital tract infection is still unknown. However, it is evident that urogenital infection can be caused by S. aureus strains which lack these genes. Other newly detected genes may play a role in pathogenesis.
bassamas's picture

Enterotoxigenic Staphylococcus Aureus in Raw Milk in the North of Palestine

Journal Title, Volume, Page: 
Turk J Biol 29, 229-232
Year of Publication: 
2005
Authors: 
Bassam ABU-SHANAB
Faculty of Veterinary Medicine, An-Najah N. University, Nablus, PALESTINE
Current Affiliation: 
Department of Animal Production and Health, Faculty of Agriculture and Veterinary Medicine, An Najah National University, Nablus, Palestine
Ghaleb ADWAN
Department of Biology and Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus
Kamel ADWAN
Department of Biology and Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus
Preferred Abstract (Original): 
Enterotoxin genes ( sea-see ) in Staphylococcus aureus isolates recovered from milk of clinically healthy sheep and cows in the north of Palestine were determined using a polymerase chain reaction (PCR). Thirty-seven (37%) out of 100 S. aureus isolates were toxin gene positive. Four strains (10.8%) were sea-positive, 20 (54.1%) were seb-positive, 4 (10.8%) were sec-positive, 6 (16.2%) were sed-positive and 3 (8.1%) were see-positive. None of these enterotoxigenic isolates carried more than one toxin gene. This study indicates that the presence of enterotoxigenic S. aureus in raw milk can contribute to the sources of staphylococcal food poisoning in Palestine.
adwang's picture

Enterotoxigenecity of S. Aureus Isolates Recovered From Chronic Urogenital Tract Infection In North Palestine

Journal Title, Volume, Page: 
Pak J Med Sci April - June 2008 (Part-I) Vol. 24 No. 2 246-250
Year of Publication: 
2008
Authors: 
Ghaleb Adwan
Faculty of Veterinary Medicine, An-Najah N. University, Nablus, Palestine
Current Affiliation: 
Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine
Bassam Abu-Shanab
Faculty Of Veterinary Medicine, An-Najah N. University, Nablus, Palestine
Kamel Adwan
Department Of Biology And Biotechnology, An-Najah N. University, Nablus, Palestine
Marwan Odeh
Al-Hikma Medical laboratories, Toulkarem, Palestine
Preferred Abstract (Original): 

Objectives: Staphylococcus aureus is an important pathogen associated with diseases in a variety of hosts including humans. It produces several toxins and virulence factors that contribute to its pathogenic potential such as staphylococcal enterotoxins (SEs). This study was conducted to determine enterotoxigenicity of S. aureus associated with chronic urogenital tract infection by detecting enterotoxin genes.
Setting: This study was done in The Microbiology laboratory, An-Najah N. University, Palestine.
Methodology: A total of 90 S. aureus isolates recovered from clinical samples from patients suffering from chronic urogenital tract infection in the North of Palestine were used to detect the presence of staphylococcal enterotoxin genes sea, seb, sec, sed and see by polymerase chain reaction (PCR) assay. Results: Out of 90 S. aureus isolates tested, it was found that 57 (63.3%) of these isolates harboured one or more enterotoxin genes. Up to 78.9% of the enterotoxigenic isolates possessed one SE gene. The majority of these enterotoxigenic strains (61.4%) isolated from both semen and urine samples harbored sec gene either alone or in combination with other genes. Also the prevalence of genes in combination was significantly more common in S. aureus isolates derived from urine 9/33 (27.3%), as compared to those derived from semen 3/24 (12.5%).
Conclusions: The role of enterotoxin genes in the pathogenesis of urogenital tract infection is still unknown. However, it is evident that urogenital infection can be caused by S. aureus strains which lack these genes. Other newly detected genes may play a role in pathogenesis.

adwang's picture

Toxigenicity of Staphylococcus Aureus Isolates From Northern Palestine

Journal Title, Volume, Page: 
Emirates Medical Journal (2006); 24(2):127-129
Year of Publication: 
2006
Authors: 
Ghaleb Mohammad Adwan
Department of Biology and Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus, Palestine
Current Affiliation: 
Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine
Bassam Ali Abu-Shanab
Faculty of Veterinary Medicine, An Najah National University, Nablus, Palestine
Kamel Mohammad Adwan
Department Of Biology And Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus, Palestine
Naser Rushdi Jarrar
Department Of Biology And Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus, Palestine
Preferred Abstract (Original): 

A total of 68 Staphylococcus aureus strains isolated from different human clinical samples in the North of Palestine were examined to detect staphylococcal enterotoxin (SE) genes A (sea), B (seb), C (sec), D (sed) and (see). Of the total isolates examined, 41.2% (28/68) were enterotoxigenic S. aureus. Twelve strains (42.9%) of enterotoxigenic S. aureus harbored seagene, ten strains (35.7%) were carried see- gene, six strains (21.4%) were positive for sec-gene. None of these enterotoxigenic S. aureus isolates harbored more than one of toxin genes. The presence of these toxin genes and other genes not be detected here might play a role in process of pathogenesis of S. aureus disease other than food poisoning but this cannot be substantiated by the results of the present study

adwang's picture

Enterotoxigenic Staphylococcus Aureus In Raw Milk In The North of Palestine

Journal Title, Volume, Page: 
STAPHTurk J Biol 29 (2005) 229-232
Year of Publication: 
2005
Authors: 
Ghaleb Adwan
Department of Biology and Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus, Palestine
Current Affiliation: 
Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine
Bassam Abu-Shanab
Faculty of Veterinary Medicine, An-Najah N. University, Nablus, Palestine
Kamel Adwan
Department Of Biology And Biotechnology, An-Najah N. University, P. O. Box (7)-Nablus, Palestine
Preferred Abstract (Original): 

Enterotoxin genes (sea-see) in Staphylococcus aureus isolates recovered from milk of clinically healthy sheep and cows in the north of Palestine were determined using a polymerase chain reaction (PCR). Thirty-seven (37%) out of 100 S. aureus isolates were toxin gene positive. Four strains (10.8%) were sea-positive, 20 (54.1%) were seb-positive, 4 (10.8%) were sec-positive, 6 (16.2%) were sed-positive and 3 (8.1%) were see-positive. None of these enterotoxigenic isolates carried more than one toxin gene. This study indicates that the presence of enterotoxigenic S. aureus in raw milk can contribute to the sources of staphylococcal food poisoning in Palestine.

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