An-Najah Staff

A total of twenty-three Echinococcus granulosus hydatid cysts were collected from infected sheep slaughtered in Nablus abattoir, Nablus – Palestine. Protoscoleces or germinal membranes were used for DNA extraction followed by PCR amplification. Amplified products were analyzed the presence of a fragment of 444 bp of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene followed by nucleotide sequencing. Overall, 21 hydatid cysts were positive compared to a negative control. The partial sequences of cox1 gene of E. granulosus strains indicated that the sheep in Palestine were infected with genotype 1 (G1), genotype 2 (G2) and genotype 3 (G3). The prevalence of these genotypes was (14/21) 66.7%, (4/21) 19.0% and (3/21) 14.3% for G1, G2 and G3, respectively. Our results showed that twelve strains of G1 belonged to the common haplotype EG01 which is the major haplotype in all the geographic populations. Phylogenetic analysis also showed that two sequences of G1 genotype which have GenBank accession No. KC109657 and KC109659 were corresponding to G1.4 micro-variants. Only the sequence of GenBank accession No. KC109652 identified in our study as G2 was found to have complete identity to the original sequence described for the cox1 gene (GenBank accession No. M84662). It is concluded that G1 genotype is the predominant genotype in sheep in Palestine. Therefore, these findings should be taken into consideration in developing prevention strategies and control programs for hydatidosis in Palestine.

Background: Community acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is a major global problem. This study attempted to investigate the prevalence of nasal carriage of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains among 360 healthy university students at An-Najah National University, Palestine. For the purpose of comparing the staphylococcal cassette chromosome methicillin resistant determinant (SCCmec) type of MRSA, 46 clinical MRSA isolates were also included in this study.
Methods: Susceptibility testing was performed by the disc diffusion method. The genetic association of MRSA isolates was investigated by SCCmec typing. A selected number of isolates were also used to amplify and sequence mecA.
Results: Nasal carriage of S aureus was found in 86 of 360 students (24%). MRSA accounted for 9% of S aureus isolates. All 86 strains of S aureus were sensitive to vancomycin. Resistance to penicillin G, amoxicillin/clavulanic acid, ciprofloxacin, erythromycin, and clindamycin was found in 98%, 93%, 33%, 23%, and 12% of the isolates, respectively. Resistance rates of the MRSA isolates were as follows: 100% resistant to penicillin G and amoxicillin/clavulanic acid, 96% to ethromycin, 52% to clindamycin, and 48% to ciprofloxacin. No vancomycin-resistant isolates were identified. In our study, nearly half (52%) of the MRSA isolates belonged to SCCmec types IVa and V. However, SCCmec types II and III are represented by 48%, whereas SCCmec type I was completely absent.ConclusionThe findings of this study indicate the existence of SCCmec type IVa in both student nasal carriers and health care settings. This emphasizes the need for implementation of a revised set of control measures in both settings. Moreover, the rational prescription of appropriate antibiotics should also be considered.

Background and Objectives: The prevalence of Post Traumatic Stress Disorders (PTSD) comorbid with Major Depressive Episodes (MDE) were explored in four areas of West Bank of Palestine in the aftermath of the second intifada. Methods: The sample consisted of 916 adult Palestinians representative of the general population. The interview was personal with the use of DSM IV criteria for PTSD and MDE (the SCID I modules). Results: The prevalence of chronic PTSD comorbid with lifetime MDE and chronic PTSD alone were found 18.7% and 26.5% respectively. Another 6.1% were diagnosed as suffering from lifetime MDE. Higher numbers of refugees were found to suffer from PTSD comorbid with MDE. The majority of respondents who reported previous suicidal behavior were comorbid cases of PTSD/MDE. The predictors differentiating between MDE alone and no diagnosis and between comorbid PTSD/MDE and no diagnosis were almost identical. Conclusions: This sample of adult Palestinians living under conditions of mass violence and continuous economic deprivation were found suffering from high rates of post PTSD and comorbid PTSD with MDE, a common finding among populations under serious traumatic exposure.

Background Community acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is a major global problem. This study attempted to investigate the prevalence of nasal carriage of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains among 360 healthy university students at An-Najah National University, Palestine. For the purpose of comparing the staphylococcal cassette chromosome methicillin resistant determinant (SCCmec) type of MRSA, 46 clinical MRSA isolates were also included in this study.
Methods Susceptibility testing was performed by the disc diffusion method. The genetic association of MRSA isolates was investigated by SCCmec typing. A selected number of isolates were also used to amplify and sequence mecA. Results Nasal carriage of S aureus was found in 86 of 360 students (24%). MRSA accounted for 9% of S aureus isolates. All 86 strains of S aureus were sensitive to vancomycin. Resistance to penicillin G, amoxicillin/clavulanic acid, ciprofloxacin, erythromycin, and clindamycin was found in 98%, 93%, 33%, 23%, and 12% of the isolates, respectively. Resistance rates of the MRSA isolates were as follows: 100% resistant to penicillin G and amoxicillin/clavulanic acid, 96% to ethromycin, 52% to clindamycin, and 48% to ciprofloxacin. No vancomycin-resistant isolates were identified. In our study, nearly half (52%) of the MRSA isolates belonged to SCCmec types IVa and V. However, SCCmec types II and III are represented by 48%, whereas SCCmec type I was completely absent.
Conclusion The findings of this study indicate the existence of SCCmec type IVa in both student nasal carriers and health care settings. This emphasizes the need for implementation of a revised set of control measures in both settings. Moreover, the rational prescription of appropriate antibiotics should also be considered. 

Abstract - A total of 68 Staphylococcus aureus strains isolated from different human clinical samples in the North of Palestine were examined to detect staphylococcal enterotoxin (SE) genes A (sea), B (seb), C (sec), D (sed) and (see). Of the total isolates examined, 41.2% (28/68) were enterotoxigenic S. aureus. Twelve strains (42.9%) of enterotoxigenic S. aureus harbored sea- gene, ten strains (35.7%) were carried see- gene, six strains (21.4%) were positive for sec-gene. None of these enterotoxigenic S. aureus isolates harbored more than one of toxin genes. The presence of these toxin genes and other genes not be detected here might play a role in process of pathogenesis of S. aureus disease other than food poisoning but this cannot be substanti- ated by the results of the present study. Keywords: Enterotoxigenic S. aureus, staphylococcal enterotoxins, SEs, Palestine.

Intra Uterine Device (IUD) has been the most popular method of contraception among Palestinian women. It is one of the very effective contraceptive methods, with failure rate ranging from 0.5% to 3% (Hatcher et al, 1989) depending on the type of IUD. One of the main problems that face women using IUD is infection Our study is a comparative study of IUD users and non-users. The study sample was from the FP clinics of M01-1 in the 4 main districts of northern West Bank. The purpose was to highlight the epidemiological pattern of IUD related infections in Northern West Bank as a representative of Palestinian women. 200 women (134 case, 66 control) were interviewed, and cervical swabs were collected and cultured for aerobic bacteria. All data were analyzed by SPSS. In general the study findings were consistent with the literature. The following statements worth reporting: The prevalence rate of isolated bacteria was higher among the IUD users than non-users (p< .05), and there was no relation to the type of IUD or duration of use in these rates.(p=.o27) Regardless of the IUD use the study findings showed alteration in the vaginal discharge among women. However this alteration was significantly higher in IUD users (58%) compared to non-users (22%).(P=. 003) The study also showed that the presence of RTI related symptoms among IUD users (61%) compared to non-users (26%) was statistically significant (p=. 000). There was no relationship between the alteration of vaginal discharge with the type of bacteria isolated in both groups (IUD users and non-users). Among the IUD users (case group), isolation of different kinds of bacteria have no significant relation with the alteration of vaginal discharge, (p=. 105), (among the non-users p=0.667).

Abstract - A total of 68 Staphylococcus aureus strains isolated from different human clinical samples in the North of Palestine were examined to detect staphylococcal enterotoxin (SE) genes A (sea), B (seb), C (sec), D (sed) and (see). Of the total isolates examined, 41.2% (28/68) were enterotoxigenic S. aureus. Twelve strains (42.9%) of enterotoxigenic S. aureus harbored seagene, ten strains (35.7%) were carried see- gene, six strains (21.4%) were positive for sec-gene. None of these enterotoxigenic S. aureus isolates harbored more than one of toxin genes. The presence of these toxin genes and other genes not be detected here might play a role in process of pathogenesis of S. aureus disease other than food poisoning but this cannot be substantiated by the results of the present study. Keywords: Enterotoxigenic S. aureus, staphylococcal enterotoxins, SEs, Palestine.

The aim of our study was to investigate the prevalence of nasal carriage of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains among 360 healthy university students at An-Najah National University, Palestine. For the purpose of comparing the staphylococcal cassette Q4 chromosome methicillin resistant determinant (SCCmec) type of MRSA, 46 clinical MRSA isolates were also included in this study. Nasal carriage of S aureus was found in 86 of 360 students (24%.) MRSA accounted for 9% of S aureus isolates. All 86 strains of S aureus were sensitive to vancomycin. Resistance to penicillin G, amoxicillin/clavulanic acid, ciprofloxacin, erythromycin, and clindamycin was found in 98%, 93%, 33%, 23%, and 12% of the isolates, respectively. Resistance rates of the MRSA isolates were as follows: 100% resistant to penicillin G and amoxicillin/clavulanic acid, 96% to ethromycin, 52% to clindamycin, and 48% to ciprofloxacin. No vancomycin-resistant isolates were identified. In our study, nearly half (52%) of the MRSA isolates belonged to SCCmec types IVa and V. However, SCCmec types II and III are represented by 48%, whereas SCCmec type I was completely absent. These findings indicate the existence of SCCmec type IVa in both student nasal carriers and health care settings. This emphasizes the need for implementation of a revised set of control measures in both settings. Moreover, the rational prescription of appropriate antibiotics should also be considered.