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PCR-Restriction Endonuclease Analysis of Mycobacterium Avium Subsp. Paratuberculosis Isolates from Goats, Sheep, and Cattle in Jordan

Journal Title, Volume, Page: 
Comparative Clinical Pathology October 2012, Volume 21, Issue 5, pp 755-760
Year of Publication: 
2012
Authors: 
Nabil Hailat
Molecular Pathology Laboratory, Department of Pathology and Animal Health, Jordan University of Science and Technology, Irbid, Jordan
Adnan Fayyad
Molecular Pathology Laboratory, Department of Pathology and Animal Health, Jordan University of Science and Technology, Irbid, Jordan
Current Affiliation: 
Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, An-Najah National University, Nablus, Palestine
Mustafa Ababneh
Department of Basic Veterinary Medical Science, Jordan University of Science and Technology, Irbid, Jordan
Wael Hananeh
Department of Basic Veterinary Medical Science, Jordan University of Science and Technology, Irbid, Jordan
Feth Eddine Rezig
Department of Basic Veterinary Medical Science, Jordan University of Science and Technology, Irbid, Jordan
Saied Jaradat
Department of Molecular Biology and Genetic Engineering, Jordan University of Science and Technology, Irbid, Jordan
Preferred Abstract (Original): 
Paratuberculosis is an endemic disease and induces high economical losses in Jordan. There is no information available on genotypic variation of Mycobacterium avium subsp. paratuberculosis (MAP) isolated from animals in Jordan. In this study, we investigated 150 fecal samples from sheep, goats, and cattle for the presence of paratuberculosis using bacterial culture and polymerase chain reaction (PCR)-RFLP analysis of insertion sequence IS1311. Analysis of the results revealed that genotypic information from sheep, goat, and cattle could classify them into cattle or sheep strains. All culture isolates from cattle, 12.5% of the isolates from sheep, and 50% of the isolates from goats were cattle strain, while 87.5% of the isolates from sheep and 50% of the isolates from goats were sheep strain. Sequencing of the IS1311 268 bp PCR product from the three animal species confirmed the different MAP patterns.

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Keratinophilic Fungi on Sheep Hairs from the West Bank of Jordan

Journal Title, Volume, Page: 
Mycopathologia. 1989 May;106(2):95-101. DOI:10.1007/BF00437087
Year of Publication: 
1989
Authors: 
M S Ali-Shtayeh
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus. Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus. Palestine
H M Arda
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus. Palestine
M Hassouna
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus. Palestine
S F Shaheen
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus. Palestine
Preferred Abstract (Original): 

The mycoflora of the hair in 285 sheep from the West Bank of Jordan was analysed and the frequency of occurrence and the relative importance value for different fungal species found were calculated. Ninety six species which belong to 36 genera were isolated. Forty one of these species were either well-known agents of animal and human mycoses (Trichophyton verrucosum, T. mentagrophytes, Microsporum nanum, M. canis, and others), or have been isolated from human and animal lesions (Arthroderma cuniculi, A. curreyi, Chrysosporium tropicum, Acremonium kiliense, Aphanoascus fulvuscens, Aspergillus versicolor, Paecilomyces lilacinus, Scopulariopsis brevicaulis, and others). These pathogenic fungi comprised 75.4% of all fungi recovered from the hair of sheep. This animal seems to represent an adequate reservoir for several dermatophytes and several potentially pathogenic fungi.

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