Paratuberculosis

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PCR-Restriction Endonuclease Analysis of Mycobacterium Avium Subsp. Paratuberculosis Isolates from Goats, Sheep, and Cattle in Jordan

Journal Title, Volume, Page: 
Comparative Clinical Pathology October 2012, Volume 21, Issue 5, pp 755-760
Year of Publication: 
2012
Authors: 
Nabil Hailat
Molecular Pathology Laboratory, Department of Pathology and Animal Health, Jordan University of Science and Technology, Irbid, Jordan
Adnan Fayyad
Molecular Pathology Laboratory, Department of Pathology and Animal Health, Jordan University of Science and Technology, Irbid, Jordan
Current Affiliation: 
Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, An-Najah National University, Nablus, Palestine
Mustafa Ababneh
Department of Basic Veterinary Medical Science, Jordan University of Science and Technology, Irbid, Jordan
Wael Hananeh
Department of Basic Veterinary Medical Science, Jordan University of Science and Technology, Irbid, Jordan
Feth Eddine Rezig
Department of Basic Veterinary Medical Science, Jordan University of Science and Technology, Irbid, Jordan
Saied Jaradat
Department of Molecular Biology and Genetic Engineering, Jordan University of Science and Technology, Irbid, Jordan
Preferred Abstract (Original): 
Paratuberculosis is an endemic disease and induces high economical losses in Jordan. There is no information available on genotypic variation of Mycobacterium avium subsp. paratuberculosis (MAP) isolated from animals in Jordan. In this study, we investigated 150 fecal samples from sheep, goats, and cattle for the presence of paratuberculosis using bacterial culture and polymerase chain reaction (PCR)-RFLP analysis of insertion sequence IS1311. Analysis of the results revealed that genotypic information from sheep, goat, and cattle could classify them into cattle or sheep strains. All culture isolates from cattle, 12.5% of the isolates from sheep, and 50% of the isolates from goats were cattle strain, while 87.5% of the isolates from sheep and 50% of the isolates from goats were sheep strain. Sequencing of the IS1311 268 bp PCR product from the three animal species confirmed the different MAP patterns.

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