Olea europaea

ralkowni's picture

Detection of olive-infecting viruses in the Mediterranean basin

Journal Title, Volume, Page: 
Proc. 4th IS on Olive Growing, Eds. C. Vitagliano & G.P. Martelli Acta Hort. 586, ISHS 2002
Year of Publication: 
2002
Authors: 
M. Saponari
Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee Via Amendola 165/A, Italy
R. Alkowni
Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee Via Amendola 165/A, Italy
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
F. Grieco
Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee Via Amendola 165/A, Italy
V. Pantaleo
Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee Via Amendola 165/A, Italy
V. Savino
Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee Via Amendola 165/A, Italy
G.P. Martelli
Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee Via Amendola 165/A, Italy
N. Driouech
Istituto Agronomico Mediterraneo, via Ceglie 9, 70010 Valenzano Bari, Italy
M. Hassan
Istituto Agronomico Mediterraneo, via Ceglie 9, 70010 Valenzano Bari, Italy
B. Di Terlizzi
Istituto Agronomico Mediterraneo, via Ceglie 9, 70010 Valenzano Bari, Italy
M.Digiaro
Istituto Agronomico Mediterraneo, via Ceglie 9, 70010 Valenzano Bari, Italy
Preferred Abstract (Original): 
Olive (Olea europaea) is host of 13 different viruses but, it is possible that other viruses, which are either non mechanically transmissible or occur in low concentration in plant tissues, are present in nature. This likelihood is supported by the widespread occurrence of double-stranded RNAs (dsRNAs) in plants negative to biological tests. As very little information is available on the geographical distribution of olive-infecting, investigations were conducted for gathering information on the prevalence and distribution of olive viruses in the Mediterranean basin based on the presence of double stranded RNA (dsRNA). A total of 527 samples were collected throughout the surveys covering 10 countries and 83 locations. Out of 286 tested samples collected from 6 Italian regions, 210 (73.4%) were dsRNA positive, whereas the average of viral infections in the Mediterranean basin was 64.5%. Molecular hybridisation tests, on 25 % of dsRNA-positive samples collected in Apulia, revealed the presence of the three nepoviruses (ArMV, CLRV and SLRSV), OLYaV and OLV-1.
Hassan's picture

Germination of 'Arabequina' Olive Seeds as Affected by Chemical Scarification, Hot Water Treatment and Endosperm Tissue

Journal Title, Volume, Page: 
Jordan Journal of Agricultural Sciences, Vol 1, No 1
Year of Publication: 
2005
Authors: 
Hassan Abu-Qaoud
Department of Plant Production and Protection, Faculty of Agriculture, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Plant Production and Protection,Faculty of Agriculture and Veterinary Medicine, An Najah National University, Nablus, Palestine
Preferred Abstract (Original): 
The effect of scarifying olive Olea europaea L. cv. Arabequina endocarp containing seeds (stony seeds) with H2SO4 and NaOH at two concentrations (0.1N and 1N) and two soaking periods (24 hrs and 48 hrs) as well as water for 24 and 48 hrs and hot water 50oC and 100oC for 24 and 84 hrs on their germination was investigated. To study the effect of the endosperm on seed germination, in vitro embryo culture of the same cultivar was performed. The germination potential of isolated embryos was compared to that of complete seeds and to sections of seeds including 2/3, 1/2, and 1/3 seed sections. The highest significantly germination percentage (60%) was obtained when the stone seeds were scarified with 0.1 N H2SO4 for 24 hrs after 115 days of planting. Treating the stony seeds with either H2SO4 for 24 hrs or water at 50o C and tap water for 24 and 48 hrs raised germination percentage over other treatments. Soaking the stony seeds with H2SO4 for 48 hrs improved germination percentage but with lower effect than H2SO4 for 24 hrs. The excised embryos and the 1/2 seed portion started to germinate after one week of culturing. The highest embryo germination percentage (70%) was recorded after one month of planting. Both 1/2 and 1/3 seed’s sections exhibited higher germination percentage than the germination of 2/3 seed section or whole seed. The results of this work indicated a pronounce effect of the endocarp and endosperm on olive seed germination.
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