diagnosis
Detection of olive-infecting viruses in the Mediterranean basin
Sun, 2015-08-23 10:08 — Raed Alkowni
Journal Title, Volume, Page:
Proc. 4th IS on Olive Growing, Eds. C. Vitagliano & G.P. Martelli Acta Hort. 586, ISHS 2002
Year of Publication:
2002
Preferred Abstract (Original):
Olive
(Olea europaea) is host of 13 different viruses but, it is possible
that other viruses, which are either non mechanically transmissible or
occur in low concentration in plant tissues, are present in nature. This
likelihood is supported by the widespread occurrence of double-stranded
RNAs (dsRNAs) in plants negative to biological tests. As very little
information is available on the geographical distribution of
olive-infecting, investigations were conducted for gathering information
on the prevalence and distribution of olive viruses in the
Mediterranean basin based on the presence of double stranded RNA
(dsRNA). A total of 527 samples were collected throughout the surveys
covering 10 countries and 83 locations. Out of 286 tested samples
collected from 6 Italian regions, 210 (73.4%) were dsRNA positive,
whereas the average of viral infections in the Mediterranean basin was
64.5%. Molecular hybridisation tests, on 25 % of dsRNA-positive samples
collected in Apulia, revealed the presence of the three nepoviruses
(ArMV, CLRV and SLRSV), OLYaV and OLV-1.
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TwitterA Survey For Fig-Infecting Viruses In Palestine
Sun, 2015-08-23 09:44 — Raed Alkowni
Journal Title, Volume, Page:
JOURNAL OF PLANT PATHOLOGY, Vol 97, No 2 (2015)
Year of Publication:
2015
Preferred Abstract (Original):
A virus disease of fig (Ficus carica) known as fig mosaic (FM) is
widely spread in Palestine, where its severity varies according to the
cultivar and the growing area. At least 10 viruses and three viroids
have been detected so far in fig trees. This study reports the results
of a preliminary survey carried out in Palestine to secure information
on the viruses associated with mosaic-infected figs. Samples were
collected from scattered trees, fig orchards and nurseries of different
areas of central Palestine, where figs are traditionally grown, and
tested for the presence of Fig mosaic virus (FMV), Fig latent virus 1
(FLV-1), Fig badnavirus-1 (FBV-1), Fig leaf mottle-associated virus 1
(FLMaV-1) and 2 (FLMaV-2), and Fig cryptic virus (FCV) in addition to
Apple dimple fruit viroid (ADFVd). The following viruses. FMV, FBV-1,
FLV-1 and FLMaV- 2, were detected by RT-PCR. FBV-1 was the most
widespread followed by FMV. The genetic diversity of FMV was assessed by
sequencing a fragment from the viral p4 protein, revealing a low
divergence from the homologous sequences from GenBank.
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