morphology

Iyad Ali's picture

Morphological Perturbation of Human Erythrocytes Exposed to Photoilluminated Riboflavin

Journal Title, Volume, Page: 
An-Najah University Journal for Research - Natural Sciences - Volume 29, Issue 1, 2015
Year of Publication: 
2015
Authors: 
Iyad A Ali
Department of Biochemistry & Genetics, Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biochemistry & Genetics, Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine
Imrana Naseem
Department of Biochemistry, Faculty of Life Sciences, AMU, Aligarh, UP, India
Preferred Abstract (Original): 

Background: Reactive oxygen species (ROS) are formed as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of oxidative stress, ROS levels can rise dramatically. This may result in significant damage to cell structures. In this work we are interested to show the effect of different ROS on the morphology of fresh human RBCs.
Methods: The RBCs were incubated with different reaction mixtures at room temperature and exposed to cool fluorescent light (800 lux). Then, cells were isolated and scanned by a scanning electron microscope.
Results:
When incubated with photoilluminated riboflavin, RBCs lost their biconcave shape and adopted a spherocytes shape. The formation of spherocytes is usually associated with spectrin deficiency. In the presence of Cu(II) and riboflavin, RBCs appeared with spikes of different sizes on their surface showing the formation of “acanthocytes”, which is usually prevalent in abetalipoprotienemia. Moreover, addition of NaN3 to riboflavin-Cu(II) system resulted in completely damaged RBCs.  Away from the above combinations, when RBCs are incubated with riboflavin-aminophylline combination, they appeared with spikes of equal lengths and sizes on their surface “echinocytes”, which usually appear in different diseases like pyruvate kinase deficiency and uremia.
Conclusion
: Red blood cells undergo different morphological changes when incubated in each of the above combinations, most probably due to the formation of different ROS and these ROS could be involved in different pathological consequences.

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sawal001's picture

Polylactide films formed by immersion precipitation: Effects of additives, nonsolvent, and temperature

Journal Title, Volume, Page: 
Journal of applied polymer science, 104(2): p. 959-971.
Year of Publication: 
2007
Authors: 
Hassan Sawalha
Food and Bioprocess Engineering Group, Wageningen University, Wageningen, The Netherlands Publication History
Current Affiliation: 
Chemical Engineering Department, An-Najah National University, Nablus, Palestine
Karin Schroën
Food and Bioprocess Engineering Group, Wageningen University, Wageningen, The Netherlands Publication History
Remko Boom
Food and Bioprocess Engineering Group, Wageningen University, Wageningen, The Netherlands Publication History
Preferred Abstract (Original): 
The influence of nonsolvent, crystallinity of the polymer film, and addition of dodecane (a poor solvent for the polymer and for the nonsolvent) on the morphology of polylactides films has been investigated and was related to phase separation behavior. Both amorphous poly-DL-lactide (PDLLA) and crystalline poly-L-lactide (PLLA) were dissolved in dichloromethane, and subsequently films were made by immersion in nonsolvent baths. PDLLA gave dense films without any internal structure, since the structure was not solidified by crystallization or glassification. PLLA films show varying structure depending on the nonsolvent. With methanol, asymmetric morphologies were observed as a result from combined liquid-liquid demixing and crystallization, while with water symmetric spherulitic structures were formed. As a next step, dodecane was added, which is not miscible with the nonsolvent, and we found it to have a strong influence on the morphology of the films. The PDLLA films with dodecane did not collapse: a closed cell structure was obtained. In PLLA films, dodecane speeds up phase separation and induces faster crystallization in the films, and the porosity, size of the pores, and interconnectivity increased. When the PLLA solutions were subjected to a heat pretreatment, crystallization could be postponed, which yielded a cellular structure around dodecane, which did not contain spherulites anymore. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 104: 959–971, 2007
sawal001's picture

Polylactide microspheres prepared by premix membrane emulsification-Effects of solvent removal rate.

Journal Title, Volume, Page: 
Journal of membrane science, 310(1-2): p. 484-493.
Year of Publication: 
2008
Authors: 
Hassan Sawalha
Food and Bioprocess Engineering Group, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands
Current Affiliation: 
Chemical Engineering Department, An-Najah National University, Nablus, Palestine
Nanik Purwanti
Food and Bioprocess Engineering Group, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands
Arjen Rinzema
Food and Bioprocess Engineering Group, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands
Karin Schroën
Food and Bioprocess Engineering Group, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands
Remko Boom
Food and Bioprocess Engineering Group, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands
Preferred Abstract (Original): 
Polylactide microspheres were prepared by pre-mix membrane emulsification and subsequent extraction of solvent in a coagulation bath, and ultimately to the gas phase. The polymer was dissolved in dichloromethane and emulsified with water or water–methanol mixtures by repeated passage through a glass membrane. During and after emulsification, the droplets are exposed to a bath consisting of a mixture of water and methanol. Transfer of dichloromethane takes place into the bath and (subsequently) to the gas phase. Compared to water, the solubility of dichloromethane is increased when using water–methanol mixtures; the continuous phase can quickly dissolve a significant amount of the solvent, while transfer to the gas phase is strongly enhanced as well. This was observed experimentally and by computer simulation, using a combined model based on the Maxwell–Stefan theory for non-ideal, multi-component mass transfer. With increasing methanol concentration, the size and span of the microspheres became smaller, and was approximately 1 μm at 30% methanol. The surface morphology of these particles was solid and smooth, whereas holes were observed in those prepared in pure water. At methanol concentrations higher than 30%, the size of the microspheres increased again. This is probably due to the swelling of the particles because of the high in-diffusion of methanol which increases the porosity of the particles. Our main conclusion is that particles of defined size and size distribution can be produced by simply adjusting the non-solvent composition of the pre-mix.
sawal001's picture

Hollow polylactide microcapsules with controlled morphology and thermal and mechanical properties

Journal Title, Volume, Page: 
AIChE Journal; 55(11): p. 2827-2834.
Year of Publication: 
2009
Authors: 
Hassan Sawalha
Food and Bioprocess Engineering Group, Wageningen University, 6700 EV Wageningen, The Netherlands
Current Affiliation: 
Chemical Engineering Department, An-Najah National University, Nablus, Palestine
Karin Schroën
Food and Bioprocess Engineering Group, Wageningen University, 6700 EV Wageningen, The Netherlands
Remko Boom
Food and Bioprocess Engineering Group, Wageningen University, 6700 EV Wageningen, The Netherlands
Preferred Abstract (Original): 
Hollow polylactide microcapsules were prepared by multistage premix membrane emulsification of polylactide/dichloromethane/oil solutions in water (nonsolvent). The effects of the different oils on the morphology, thermal, and mechanical properties of the hollow microcapsules were investigated. All oils resulted in hollow microcapsules with controlled shell thickness of ∼60 nm except for eugenol, in which irregular, massive capsules were obtained. The properties of the microcapsules were strongly dependent on the oil used, for example the thermal transition temperatures found for hollow capsules were lower than for solid particles prepared without any oil. The crystallinity and transition temperatures of the capsules prepared with linear alkanes were higher than for cyclic alkanes; terpenes gave the lowest transition temperatures. The shell stiffness, measured with atomic force microscopy, was highly dependent on the oil used. Capsules prepared with dodecane showed higher stiffness (3.3 N m−1) than with limonene (2 N m−1) or cyclohexane (1.4 N m−1). © 2009 American Institute of Chemical Engineers AIChE J, 2009
samibdir's picture

Two New Cercariae From Melanoides Tuberculata (Müller) Snails In Jordan

Journal Title, Volume, Page: 
Helminthologia, vol. 25, no2, pp. 139-145 (20 ref.)
Year of Publication: 
1988
Authors: 
N. S. Ismail
Yarmouk univ., dep. biological sci., Irbid, Jordan
S. M. Bdair
Yarmouk univ., dep. biological sci., Irbid, Jordan
Current Affiliation: 
Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine
I. Issa
Yarmouk univ., dep. biological sci., Irbid, Jordan
Preferred Abstract (Original): 

Two new forms of cercariae are described from the fresh water snail Melanopsis tuberculata collected from Azraq Oasis and East Ghor Canal, Jordan during 1983 and 1985, respectively. Cercaria sp. I is a xiphidiocercaria belonging to the "Pusilla" sub-type. It has 3 pairs of penetration glands and a flame cell formula 2[(2+3+1+2)+(2)]= 20.Cercaria sp. II is an ocellate, apharyngeal brevifurcate cercaria. It has 5 pairs of penetration glands and a flame cell formula 2[(1+1+1)+(1+1)+(1)]= 12. Both cercariae develop in sporocysts

samibdir's picture

Two New Furcocercariae From Melanopsis Praemorsa (L., 1875) (Thiaridae) Snails In Jordan

Journal Title, Volume, Page: 
Hilminthologia, vol. 26, no.1, pp. 15-20 [6 page(s) (article)] (8 ref.)
Year of Publication: 
1989
Authors: 
N. S. Ismail
Yarmouk univ., dep. biological sci., Irbid, Jordan
S. M. Bdair
Yarmouk univ., dep. biological sci., Irbid, JORDANIE
Current Affiliation: 
Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine
Preferred Abstract (Original): 

Two new forms of furcocercariae are described from the fresh water snail Melanopsis praemorsa collected from Menshiya Station at Jordan River and Jarash Falls, Jordan during 1984 and 1985. One is pharyngeate longifurcate monostome cercaria and the second is pharyngeate longifurcate distome cercaria. These cercariae are named Cercaria melanopsis XIV and XV. Both forms of cercariae develop within long thread-like sporocysts. Details are presented on the morphology and behaviour of these cercariae

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