genotypes

motasem's picture

Respiratory Syncytial Virus Infection in Hospitalized Children Older Than 2 Years with Community-Acquired Pneumonia

Journal Title, Volume, Page: 
HIPPOKRATIA, 17, 2:146-149
Year of Publication: 
2013
Authors: 
M Almasri
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
A Papa
Department of Microbiology of Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece
M Eboriadou
Fourth Department of Paediatrics of Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece
Souliou E
Department of Microbiology of Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece
Haidopoulou K
Fourth Department of Paediatrics of Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece
Preferred Abstract (Original): 

Background/aim: Respiratory syncytial virus (RSV) is one of the main causes of bronchiolitis and pneumonia in infants and young children. The aims of the present study were to evaluate the role of RSV in children >2 years old hospitalized with community-acquired pneumonia (CAP) and to type the circulating RSV strains. Materials and Methods: Serum and throat swab samples were taken upon admission from Greek children aged > 2 years, hospitalized with atypical CAP, and when possible, a second serum sample was also taken. RSV IgG and IgM antibodies were determined by Enzyme Linked Immunosorbent Assay (ELISA), while throat swab samples were tested by nested RT-PCR. Additional serological testing was performed to find out probable co-infections.
Results: A total of 101 children with atypical CAP were included in the study, aged 2.5-14 years (median 8.25). RSV IgM antibodies were detected in 21 (20.7%) cases, either in the first or/and in the second serum sample, while RSV genome was detected in 11 out of 15 (73%) IgM-positive patients, which were further tested by PCR. PCR-positive results were obtained up to the 7th day of illness. Among the 11 cases, one was of type B, and all the rest were of type A. The median age of the RSV-positive children was 4 years (range 3-13 years). Although RSV was detected in all seasons, the majority of cases (31%) were detected in winter. Co-infection was detected in 3 cases (two with Mycoplasma pneumoniae and one with adenovirus).
Conclusions: Apart from the known role of RSV as the most important pathogen causing acute respiratory disease in infants and young children, it is also a significant viral pathogen in older children hospitalized because of CAP. Genetic typing provides further insight into the epidemiology of the disease.

mshtaya's picture

Genetic Variability of Some Palestinian Fig (Ficus Carica L.) Genotypes Based on Pomological and ‎Morphological Descriptors ‎

Journal Title, Volume, Page: 
An-Najah University Journal for Research (Natural Sciences), 27 (1)
Year of Publication: 
2013
Authors: 
Munqez Shtaya
Department of Plant Production & Protection, An-Najah University, Palestine.
Current Affiliation: 
Department of Plant Production and Protection,Faculty of Agriculture and Veterinary Medicine, An Najah National University, Nablus, Palestine
Rezq Basheer-Salimia
Department of Plant Production & Protection, Faculty of Agriculture, Hebron University
Murad Awad
Department of Plant Production & Protection, Faculty of Agriculture, Hebron University
Yamen Hamdan
Palestine Technical University-Kadoorie
Preferred Abstract (Original): 
Characterization of fruit species is recognized as a primary and essential step towards protecting, conserving, maintaining, and conducting any future breeding program. The aim of this study is the genetic characterization of twelve fig genotypes (Ficus carica L.) from the southern region of the West Bank, Palestine, based on 41 pomological and morphological traits developed by IPGRI and CIHEAM (2003) with some minor modifications. Results showed a considerable diversity among all tested genotypes at both pomological and morphological levels. UPGMA dendrogram clustered the genotypes into four clusters (Fig. 1). The first (I) consisted of one genotype (Khdari). The second cluster (II) consisted of four genotypes (Ghzali, Bladi, Shhami and Hmari). The third cluster (III) consisted of four genotypes (Mwazi, Moozi, Ruzzi and Mouze). The fourth cluster (IV) consisted of three genotypes (Aswad, Swadi and Smari). Genetic distances ranged from 0.517 to 0.863 with a mean of 0.690. “Ruzzi and Mouze” were the most closely related genotypes, followed by “Ruzzi and Moozi”; “Moozi and Mouze”; “Mwazi and Ruzzi” and “Swadi and Smari”. In contrary, “Khdari and Aswad” and Ghzali and Aswad” were the most distantly related ones.
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