2013/2014
An-Najah National University
Faculty of Science–Biology and Biotechnology Dept.
Special Topics 24481 |
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Instructor(s) name(s) |
Dr. Raed Alkowni |
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Contact information |
ralkowni@najah.edu, office : 14F1170 |
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Fall Semester 2013/2014 |
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Compulsory / Elective |
Department Elective Course |
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Prerequisites |
24102 & 24108 |
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Course Description (Content) |
Various up-to-date topics on biology and biotechnology |
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Course Objectives |
To provide well understanding with the recent advances on molecular biology, genetic engineering; plant pathology and advances on molecular diagnostic technologies. At the end the each session students will be able to critically evaluate recently published research papers on abovementioned topics. |
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Intended learning Outcomes (ILO’s) and Competences
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At the end of this course student will be able to: 1. illustrate the principle and function of immune system warm blood animals; 2. apply different serological techniques for diagnostic purposes. 3. contrast up-to-date molecular tools for diagnosis and advanced genetic sequencing techniques to reveal molecular diversity of organisms 4. clarify and compare gene transfer methodologies in transgenic organisms 5. explain how various bio-techniques can be used for molecular profiling of organisms as well as for forensics. 6. argue the ethical issues related to GMOs use 7. clarify virus’ replications with special focus on phyto-viruses. 8. explain how Genes are expressed; regulated and technologies that can be used as a tool for measuring that. 9. List the available opportunities in biotechnology as a career 10. Present, evaluate and discuss independently research papers on topics of cell biology as apoptosis and oncology in the class. |
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Textbook & References
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· Analysis of genes and genomes. 2004. Richard J. Reece. Ed. Wiley. · Cell and molecular Biology, Concepts and Experiments, 5rd Edition. 2007. Gerald Karp. John Wiley and Sons, Ins. · iGenetics: A Molecular Approach (3rd edition) 2010. Peter J. Russell. · Introduction to Plant biotechnology 2nd Ed. 2002. Chawla. Science Publishers, Inc. USA. · Molecular Biotechnology: principles and applications of recombinant DNA 3rd Ed. 2003. Glick, B.R. and Pasternak, J.J. ASM Press, Washington DC. · NCBI handbook. 2003. The national library of Medicine. · Plant Biotechnology in Agriculture. 1989. K. Lindsey and MGK Jones. Milton Keynes: Open University Press (1989), · Molecular Biology of the Gene (5th ed) - JD Watson – 2004 · Up-to-date research papers |
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Teaching Methods |
- Interactive presentation - Individual reflection and writing - Small group work - Large group discussion |
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Assignment Criteria |
Activity |
Percent (%) |
10 |
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First Exam |
20 |
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Second Exam |
20 |
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Final Exam |
50 |
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Total |
100 |
Course outline
Week no. |
Selected Topic |
1-2 |
The Immune system |
3 |
Serology as a diagnostic tool -Monoclonal Production -Polyclonal Production -ELISA |
4-5 |
Advanced DNA-based diagnostic techniques [i.e: Real time PCR; Taqman; Low density PCR; nested PCR; IC-PCR,RT-PCR] |
First Exam |
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6 |
Deep sequencing techniques |
7-8 |
Molecular Markers for measuring genetic biodiversity |
9 |
Advances in Forensic applications |
10 |
Gun (Biolistic method) as a technique for genetic transformations; Bioethics and legislation related to the use of genetic modified organisms |
Second Exam |
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11-12 |
Virology: virus replications, ssRNA viruses, Plant viruses, Sanitation |
13 |
Natural enemies and biocontrol |
14 |
Expressed genes profiling (Microarrays) |
15 |
Epigenetics |
16 |
Career in Biotechnology |
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Final Exam |