Genetic Diversity Among Palestinian Fig Varieties (Ficus Carica L.) Using ISSR, And RAPD Markers

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Ayat Khaled Mubaslat
Genetic_Diversity_among_Palestinian_Fig_Varieties_(Ficus_Carica_L.)_Using_ISSR,_and_RAPD_Markers_.pdf5.12 MB
The genetic diversity in Palestinian fig (Ficus carica L.), was studied using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. Twenty three fig accessions (20 common fig cultivars, and two more wild types and one San pedro type (Dafor) crossponding to the main cultivated varieties in Palestine were analyzed. The cultivars were assessed using the randomly amplified polymorphic DNA (RAPD) technique. The 21 out of 25 screened markers showed reproducible polymorphic profiles. The generated 1518 data entries were analyzed among which 420 entries were for present bands and 1098 for absent bands. After determining Jaccard similarity index, some genotypes showed high genetic similarity (77%) was recorded between Zraqi and Ghzali, while other were less similar (0% between Ajloni and Qraee). Moreover, the primers were evaluated for their resolving power, where primer OPH08 achieved the highest power 3.16 meanwhile the weakest power was shown by primer OPA03 (0.15). Furthermore , dendrogram was elaborated by cluster analysis according to the UPGMA algorithm . The genotypes were clustered into seven clades . The mean number of amplification RAPD bands (6.7) was little more than that of ISSR (5.57). Moreover, the total number of polymorphic bands (161) detected by RAPD primers was much higher than that of the ISSR primers (69), which suggested that the RAPD markers were more powerful compared to ISSR markers in the capacity of revealing more informative bands in a single amplification.