Biological Effects of 6 mT Static Magnetic Fields: A Comparative Study in Different Cell Types

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Journal Title, Volume, Page: 
Bioelectromagnetics Volume 27, Issue 7, pages 560–577, October 2006
Year of Publication: 
2006
Authors: 
Bernadette Tenuzzo
Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, Italy
Alfonsina Chionna
Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, Italy
Elisa Panzarini
Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, Italy
Remigio Lanubile
Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, Italy
Patrizia Tarantino
Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, Italy
Bruno Di Jeso
Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, Italy
Majdi Dwikat
Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, Italy
Current Affiliation: 
Department of Medical Laboratory Sciences, Faculty of Science, An-Najah National University, Palestine
Luciana Dini
Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, Italy
Preferred Abstract (Original): 

The present work was a comparative study of the bio-effects induced by exposure to 6 mT static magnetic field (MF) on several primary cultures and cell lines. Particular attention was dedicated to apoptosis. Cell viability, proliferation, intracellular Ca2+ concentration and morphology were also examined. Primary cultures of human lymphocytes, mice thymocytes and cultures of 3DO, U937, HeLa, HepG2 and FRTL-5 cells were grown in the presence of 6 mT static MF and different apoptosis-inducing agents (cycloheximide, H2O2, puromycin, heat shock, etoposide). Biological effects of static MF exposure were found in all the different cells examined. They were cell type-dependent but apoptotic inducer-independent. A common effect of the exposure to static MF was the promotion of apoptosis and mitosis, but not of necrosis or modifications of the cell shape. Increase of the intracellular levels of Ca2+ ions were also observed. When pro-apoptotic drugs were combined with static MF, the majority of cell types rescued from apoptosis. To the contrary, apoptosis of 3DO cells was significantly increased under simultaneous exposure to static MF and incubation with pro-apoptotic drugs. From these data we conclude that 6 mT static MF exposure interfered with apoptosis in a cell type- and exposure time-dependent manner, while the effects of static MF exposure on the apoptotic program were independent of the drugs used. Bioelectromagnetics 27:560–577, 2006. © 2006 Wiley-Liss, Inc.

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