An-Najah Staff

Abstract: The effect of different levels of auxin Naphthaleneacetic acid (NAA) and cytokinin Benzyl adenin (BA) on shoot regeneration of Petunia hybrida was studied in these experiments. The regenerated shoots were grown for somaclonal variation. Seeds of Petunia hybrida_were in vitro planted onto MS basal medium. The growing seedlings were used as a source material for the regeneration experiments. Four levels of BA (0, 0.1, 0.4, 0.8 mg/1) and two levels of NAA (0.0, 0.1 mg/1) were used with MS basal media. Two sources of explants were used; leaf sections and shoots. Low regeneration percentage was achieved with leaf explants. The highest shoot number (1.7) was obtained with MS medium supplied with 0.4 mg/1 BA and 0.1 mg/I NAA. However, in case of shoot explant higher shoot number was obtained with 0.8 mg/I BA and 0.1 mg/1 NAA. For somaclonal variation, buds were taken from petunia plants of pink color that were grown in the green house. The buds were disinfected and grown on MS basal media supplied with 30 mg/1 Gentamycin sulfate and 30 mg/I Benlate, after shoot growth they were transferred onto the shoot regeneration medium. The regenerates were rooted, acclimatized and transferred to the green house for evaluation. Two forms of leaf shape orbicular and elliptic and three flower colors violet, purple and light pink were appeared with the plants.

The effect of different levels of naphthaleneacetic acid (NAA) and benzyladenine(BA) on shoot multiplication and regeneration of Petunia hybrida was studied. Regenerated shoots from leaf explants were grown ex vitro for analysis of somaclonal variation. Seeds of Petunia hybrida were germinated in vitro on MS basal medium. The seedlings were used as a source of explants for the multiplication and regeneration experiments. For the shoot multiplication experiment, shoot explants taken from germinated seedlings were cultured on MS basal media supplemented with different concentrations of BA (0.1, 0.4, 0.8 mg l−1) and NAA at concentration of0.1 mg l−1. Highest number of axillary shoot was obtained on medium supplemented with 0.8 mg l-1 BA and 0.1 mg l−1NAA. For regeneration experiments, leaf sections taken from germinated seedlings were cultured onto MS media supplemented with three levels of BA (0.5, 1.0 and 2.0 mg l−1) and 0.5 mg l−1NAA. The highest shoot regeneration rate (45%) was observed in MS medium supplemented with 2 mg l−1 BA. For the evaluation of somaclonal variation, lateral bud explants were taken from pink colored petunia plants that were grown in the greenhouse. The buds were disinfected and cultured on MS basal media supplied with 30 mg l−1 gentamycin sulfate and 30 mg l−1 Benlate. After shoots grew, leaf sections were then taken from them shoots and cultured onto shoot regeneration medium (MS medium supplemented with 2 mg l−1 BA). The regenerated adventitious shoots were cultured on MS medium without growth regulator. These shoots were then rooted, acclimatized and transferred to the greenhouse for evaluation. Two forms of leaf shape (orbicular and elliptic) and three flower colors (violet, purple and light pink) appeared on the plantlets.