Palestinian snake melon landraces, Genetic diversity, Cucumis melo var. flexuosus, Population structure, SNP, Genotyping by sequencing, DArT-seq

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Characterizing Palestinian snake melon (Cucumis melo var. flexuosus) germplasm diversity and structure using SNP and DArTseq markers

Journal Title, Volume, Page: 
BMC Plant Biology
Year of Publication: 
2018
Authors: 
Salam Y. Abu Zaitoun
Biodiversity and Environmental Research Center, BERC, Til, Nablus, Palestine
Rana M. Jamous
Biodiversity and Environmental Research Center, BERC, Til, Nablus, Palestine
Munqez J Y Shtaya
Department of Plant Production and Protection, Faculty of Agriculture and Veterinary Medicine
Omar B. Mallah
Biodiversity and Environmental Research Center, BERC, Til, Nablus, Palestine
Imad S. Eid
Biodiversity and Environmental Research Center, BERC, Til, Nablus, Palestine
Mohammed S. Ali-Shtayeh
Biodiversity and Environmental Research Center, BERC, Til, Nablus, Palestine
Preferred Abstract (Original): 
Background: Crop landraces embody a source of beneficial genes potentially providing endurance to environmental
stress and other agronomic qualities including yield. Our study included 88 snake melon accessions (Cucumis melo var.
flexuosus) collected from 9 districts in the Palestinian West-Bank. These accessions represent four landraces of Palestinian
snake melon: Green, and White Baladi, and Green, and White Sahouri.
Results: This is the first report on successful application of genotyping by sequencing in snake melon. Nine thousand
seven hundred fifty single-nucleotide polymorphism (SNP) and 7400 DArTseq genetic markers were employed
to evaluate genetic biodiversity and population structure of Palestinian snake melon germplasm collection. Clustering
based on neighbor-joining-analysis, principle coordinate and Bayesian model implemented in Structure showed that
patterns of genetic diversity of snake melon landraces depends on their geographical source and unraveled the
presence of two major local landraces (Sahouri, and Baladi) with accessions from each group clustering together. A
significant correlation was observed between both types of markers in Mantel correlation test. A significant association
between genetic and geographic matrices (P < 0.0001) was also detected. AMOVA indicated that majority of variation
(90%) was due to the difference within accessions.
Conclusion: The Palestinian landraces seem to have unique genes that may allow the enhancement of the global
snake melon gene pool and developments of the plant production worldwide. Our subsequent objective is to detect
genotypes with promising qualities and to conduct association mapping studies concentrating on Fusarium-wilt
resistance, yield, and environmental stresses.
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