Oxygen radicals

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Hemolysis of Human Red Blood Cells by Combination ‎of Riboflavin and Aminophylline

Journal Title, Volume, Page: 
Life Sciences, 70, 2013-2022, USA
Year of Publication: 
2002
Authors: 
Iyad A.F. Ali
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, India
Current Affiliation: 
Department of Biomedical Sciences, Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine
Naseem I
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, India
Preferred Abstract (Original): 

The effect of aminophylline on human red blood cells (RBC) has been studied. Under in vitro condition, aminophylline alone does not hemolyse RBC. However, in the presence of riboflavin and visible light, aminophylline causes hemolysis of RBC. This hemolysis depends on the concentration of both riboflavin and aminophylline. Using different free radical scavengers we show that RBC hemolysis is caused by reactive oxygen species. Studies using bovine serum albumin show that riboflavin-aminophylline combination can also cause protein degradation in vitro.

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