liquid chromatography

Nidal Zatar's picture

Simultaneous Determination of Seven Synthetic Water-Soluble Food Colorants by Ion-Pair Reversed-Phase High-Performance Liquid Chromatography

Journal Title, Volume, Page: 
Journal of Food Technology, 5 (3) (2007)20-224.
Year of Publication: 
2007
Authors: 
Nidal A. Zatar
Chemical, Biological and Drug Analysis Center, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Chemistry, An-Najah N. University, PO Box 7, Nablus, West Bank, Palestine
Preferred Abstract (Original): 

A selective gradient ion-pair reversed-phase high-performance liquid chromatographic method for the simultaneous quantitative determination of seven synthetic water-soluble food colorants (Tartrazine E102, Quinoline Yellow E104, Sunset Yellow E110, Carmoisine E122, Ponceau 4R E124, Erythrosine E127, Carmine E132 and Brilliant Blue FCF E133) was developed. Analysis were performed on 125×4.6 mm i.d. Merck Lichrosher 100 RP C-18 (5 µm ) column. The flow rate of the mobile phase was 1.0 mL min 1 and the injection volume was 50 µL. The mobile phase consisted of water:acetonitrile (50:50 v v 1) Containing 0.35 M (1-Hexadecyl) Trimethylammonium Bromide (CTAB) and buffered to pH 7.5 (mobile phase A) and water acetonitrile (50:50 v v 1) (mobile phase B). Successful separation and quantitative determination was obtained for all the colorants using an optimized gradient elution program. Detection limits were 1.59 (E142) and 22.1 (E124) ppm, with relative standard deviations in the range 0.37%. The method was applied to the determination of colorants in various types of drinks and food, such as carbonated fruit flavored drinks, concentrated fruit flavored drinks, jams and sugar confectionery.

Nasr SHRAIM's picture

Determination of Reboxetine in Rat Brain Microdialysates and Plasma Samples Using Liquid Chromatography Coupled To Fluorescence Detection

Journal Title, Volume, Page: 
Journal of Chromatography B Volume 898, 1 June 2012, Pages 53–61
Year of Publication: 
2012
Authors: 
Naser Shraim
Vrije Universiteit Brussel, Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences, Laarbeeklaan 103, B-1090 Brussels, Belgium
Current Affiliation: 
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Ralph Clinckers
Vrije Universiteit Brussel, Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences, Laarbeeklaan 103, B-1090 Brussels, Belgium
Sofie Sarre
Vrije Universiteit Brussel, Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences, Laarbeeklaan 103, B-1090 Brussels, Belgium
Yvette Michotte
Vrije Universiteit Brussel, Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences, Laarbeeklaan 103, B-1090 Brussels, Belgium
Ann Van Eeckhaut
Vrije Universiteit Brussel, Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences, Laarbeeklaan 103, B-1090 Brussels, Belgium
Preferred Abstract (Original): 
A liquid chromatographic method with fluorescence detection was developed and validated for the quantification of the antidepressant reboxetine (RBX), a selective noradrenalin reuptake inhibitor, in rat brain microdialysates. After modification of the method in terms of sample preparation and sensitivity, it was also validated for the quantification of RBX in rat plasma samples. To enable fluorescence detection, a pre-column derivatization step with 9-fluorenylmethyl chloroformate was included. Separations were performed on a reversed phase C₁₈ column using gradient elution. The retention time for RBX was found to be 8.8 min. The assay of RBX in brain microdialysis samples showed a linear relationship in the calibration curve from 2 to 200 ng/mL, with a correlation coefficient ≥0.999. The limit of detection (LOD) and the lower limit of quantification (LLOQ) were 0.6 and 2.0 ng/mL respectively. The intra-day and the inter-day precision (RSD %) ranged between 1.5% and 11.7% with an average recovery of 101.2±8.2% (mean±SD, n=40). For the analysis of plasma samples, the calibration curve was linear between 20 and 700 ng/mL with a correlation coefficient ≥0.999. LOD and LLOQ were 6 and 20 ng/mL respectively. The intra-day and the inter-day precision (RSD %) ranged between 1.7% and 11.5% with an average recovery of 98.5±7.3% (mean±SD, n=40). We demonstrated the applicability of the method to determine the concentration-time profiles of RBX in brain and plasma following systemic administration.
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