An-Najah Staff

In the present study a simple isocratic reversed-phase high-performance liquid chromatography method has been developed and validated for the assaying of desferrioxamine released from nanoparticles in buffer solution (pH=7.4). Desferrioxamine was determined in its iron(III) complex form (ferrioxamine). The analytical column, nucleosil C8 (5 µm particle size, 250 mm?4.6 mm I.D.), was operated at 40°C. Isocratic elution was performed using a mobile phase consisted by 27 mM KH2PO4/tetrahydrofuran/trietylamine (93:7:0.05 v/v/v), at a flow rate of 1.2 mL/min. UV detection was performed at 430 nm. The method was linear in the 0.02-20 µg/mL ferrioxamine concentration range (r2 = 0.9998). Intra-day precision ranged from 1.32 to 4.37% and accuracy was better than 99.6%, while concerning inter-day precision this was ranged from 1.73 to 4.69% and accuracy was better than 99.2%. The method was found to be robust while performing small changes in column temperature (± 2°C), flow rate of mobile phase (± 0.2 mL/min), buffer’s pH of mobile phase (± 0.5) and %THF content of mobile phase (± 2.5%), with accuracies varying between 99.0 and 105.0 % in most cases. Selectivity of the method was confirmed by the absence of interference of blank samples. Stability of ferrioxamine at temperatures 4°C, 25°C and 37°C was also studied and was found to be stable even after eight days.