carbohydrates

m.d.assali's picture

Improved Non-Covalent Biofunctionalization of MWCNTs using Carbohydrates Amphiphiles with a Butterfly-like Polyaromatic Tail

Journal Title, Volume, Page: 
Nano research, 3, 764
Year of Publication: 
2010
Authors: 
mohyeddin assali
Instituto de Investigaciones Químicas, C.S.I.C-Universidad de Sevilla, c/. Américo Vespucio, 49, Isla de la Cartuja, Sevilla 41092, Spain
Current Affiliation: 
Department of pharmacy, faculty of medicine and health sciences, An Najah National University, Nablus, Palestine
Manuel Pernía Leal
Instituto de Investigaciones Químicas, C.S.I.C-Universidad de Sevilla, c/. Américo Vespucio, 49, Isla de la Cartuja, Sevilla 41092, Spain
Inmaculada Fernández
Departamento de Química Orgánica y Farmacéutica, Facultad de Farmacia, Universidad de Sevilla, Sevilla 41012, Spain
Pablo Romero-Gomez
Instituto de Ciencias de Materiales de Sevilla, C.S.I.C-Universidad de Sevilla, c/. Américo Vespucio, 49, Isla de la Cartuja, Sevilla 41092, Spain
Rachid Baati
Université de Strasbourg Faculté de Pharmacie CNRS/UMR 7199, Laboratoire des Systèmes Chimiques Fonctionnels BP 60024, 74 route du Rhin, 67400 Illkirch, France
noureddine Khiar
Instituto de Investigaciones Químicas, C.S.I.C-Universidad de Sevilla, c/. Américo Vespucio, 49, Isla de la Cartuja, Sevilla 41092, Spain
Preferred Abstract (Original): 
ugar-based amphiphiles functionalized with the butterfly-like tetrabenzo[a,c,g,i] fluorene undergo stronger π–π interactions with multi-walled carbon nanotubes than amphiphiles with pyrene tails. The resulting water soluble nanoglycoarrays with a multivalent sugar exposition on their surface engage in specific ligand–lectin interactions similar to glycoconjugates on a cell membrane.
m.d.assali's picture

Copper-Catalyzed Azide–Alkyne Cycloaddition in the Synthesis of Polydiacetylene: “Click Glycoliposome” as Biosensors for the Specific Detection of Lectins.

Journal Title, Volume, Page: 
Chemistry- a European journal, 17, 1828
Year of Publication: 
2011
Authors: 
manuel pernia leal
Department of Bioorganic Chemistry, CSIC-Universidad de Sevilla, Américo Vepucio, 49, Isla de la Cartuja, 41092 Seville (Spain)
mohyeddin assali
Department of Bioorganic Chemistry, CSIC-Universidad de Sevilla, Américo Vepucio, 49, Isla de la Cartuja, 41092 Seville (Spain)
Current Affiliation: 
Department of pharmacy, faculty of medicine and health sciences, An Najah National University, Nablus, Palestine
Inmaculada Fernández
Department of Organic and Pharmaceutical Chemistry, University of Seville, c/ García González, 12, 41012 Seville (Spain)
Noureddine khiar
Department of Bioorganic Chemistry, CSIC-Universidad de Sevilla, Américo Vepucio, 49, Isla de la Cartuja, 41092 Seville (Spain)
Preferred Abstract (Original): 

Supramolecular self-assembly of conjugated diacetylenic amphiphile-tethered ligands photopolymerize to afford polydiacetylene (PDA) functional liposomes. Upon specific interaction with a variety of biological analytes in aqueous solution, PDA exhibits rapid colorimetric transitions. The PDA nanoassemblies, which are excellent membrane mimics, include an ene–yne polymeric reporter responsible for the chromatic transitions and the molecular recognition elements that are responsible for selective and specific binding to the biological target. A bottleneck in the fabrication of these colorimetric biosensors is the preparation of the diacetylenic monomer embedded with the recognition element of choice. In the present work, we make use of copper-catalyzed azide–alkyne cycloaddition (CuAAC) as key step in the preparation of sugar-coated liposome biosensors. The regioselective click ligation of the triacetylenic N-(2-propynyl)pentacosa-10,12-diynamide (NPPCDAM) with a variety of mannose- and lactose-tethered azides afforded chemo- and regioselectively the corresponding 1,2,3-triazole. The obtained diacetylenic monomers were incorporated efficiently into vesicles to afford functional mannose- and lactose-coated glycoliposomes. The obtained PDA-based click glycoliposomes have been characterized by using transmission electronic microscopy (TEM), dynamic light scattering (DLS), and UV/Vis spectroscopy. The efficiency of the reported approach was demonstrated by the rapid optimization of the hydrophilic spacer between the lipidic matrix and the mannose head group for the colorimetric detection of Concavalin A.

Syndicate content