An-Najah Staff

 

 

 

An-Najah N. University

COURSE OUTLINE

BIOL24341- GENERAL MICROBIOLOGY

SPRING SEMESTER 2010           

COURSE CATALOG DESCRIPTION: Morphology, physiology, classification, and cultivation of bacteria and viruses, with introduction to microbial genetics, pathogenesis, and immunology.

PREREQUISITES: BIOL 24102, 24108

                                                                                                                                                        

CREDIT: 4                                                                                                                                    

                                                                                                                                                        

INSTRUCTOR: Associate professor Kamel Adwan

  

REQUIRED MATERIALS:

 

1. Textbook: Microbiology an Introduction by Tortora-Tenth edititon-2010

2. Lab Book: Laboratory experiments in Microbiology, by Johnson.

                                                                                                                                                                                          

METHODS OF INSTRUCTION: Formal lecture, discussion, instructor-prepared handouts, laboratory exercises, various audiovisual presentations.                                                                                  

                                                                                                                                                        

METHODS OF EVALUATION (Grading Policy):                                                                    

 

4 Lecture exams:       Exam format is mostly objective (multiple choice, T/F, matching occasional short-answer questions. Exams are announced at least one week in advance.

First Exam: 20%. Second Exam: 20%. Lab work: 25%. Final Exam: 35%.

 

Laboratory quizzes:  Written quizzes will follow most every four or five laboratory sessions and will be worth approximately 25 points each. The laboratory portion of the course will make up approximately 25% of the course grade.

                                                                                                                                                                

Course Outline/Weekly Schedule

 

1.   The Microbial World

2. Observing Microorganisms through Microscope

 

3 and 4. Functional anatomy of prokaryotic cells:

Comparing of prokaryotic and eukaryotic cells

Prokaryotic cells: size, shape and arrangement

Structure external to the cell wall

The cell wall

Structure internal to the cell wall

5 and 6. Microbial growth and reproduction:

Requirements for growth

Culture media

Preserving bacterial cultures

The growth of bacterial culture

7. The control of microbial growth:

Principle of microbial control

The rate of microbial death

Actions of microbial control agents

Physical methods of microbial control

Chemical methods of microbial control

8and 9. Microbial Genetics

Mutation: change in the genetic material

Genetic transfer and recombination

10 and 11. Viruses

General characteristics of viruses

Viral structure

The isolation, cultivation and identification

Viral multiplication

12 and 13. Microbial mechanisms of pathogenicity

Entry of microorganisms into the host

How bacterial pathogens penetrate host defenses

How bacterial pathogens damage host cells

14 and 15. Antimicrobial drugs

The spectrum of antimicrobial activity

The action of antimicrobial drugs

Tests to guide chemotherapy

The effectiveness of chemotherapeutic agents

16. Practical applications of immunology

The nature of antigens and antibodies and diagnostic immunology:

THE ABOVE SCHEDULE AND PROCEDURES IN THIS COURSE OUTLINE ARE SUBJECT TO CHANGE IN THE EVENT OF EXTENUATING CIRCUMSTANCES.

 

MICROBIOLOGY LABORATORY SCHEDULE - SPRING 2006

1. Use and care of the microscope

2. Smear preparation and simple staining

3. Gram stain

4. Special stains

5. Pure culture technique

6. Culture media and culturing microorganisms from the environment

7. Moist and dry heat; Autoclave

8. Protozoans and Helminths

9. Biochemical Tests

10. The effects of ultraviolet light

11. The use of antiseptics and disinfectants

12. Diagnostic bacteriology

13. Antibiotics

14. Serology

Note:The above laboratories are not necessarily in chronological order. What labs we will do will be announced ahead of time. I expect students to read the labs ahead of time.