Emodin, A Naturally Occuring Anthraquinone: Its Isolation and Spectrophotometric Determination in Rumex Cyprius Plant

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Journal Title, Volume, Page: 
Spectroscopy Letters: An International Journal for Rapid Communication Volume 29, Issue 8
Year of Publication: 
1996
Authors: 
Waheed J. Al-Jondi
Chemistry Department, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
Mohammed A. Al-Nuri
Chemistry Department, An-Najah National University, Nablus, Palestine
Nidal A. Za'tar
Chemistry Department, An-Najah National University, Nablus, Palestine
Maher A. Abu-Eid
Chemistry Department, An-Najah National University, Nablus, Palestine
Mohammad A. Hannoun
Chemistry Department, An-Najah National University, Nablus, Palestine
Mohammad S. Ali-Shtayeh
Biology Department, An-Najah National University, Nablus, Palestine
Preferred Abstract (Original): 

A new method for isolation and specrophotometric determination of emodin is presented. Emodin was isolated by thin layer chromatography (tlc) and column chromatography (cc) techniques, as an orange long crystalline substance. Emodin exhibits two absorption maxima, at 420 and 520 nm. Stability of the color and the effect of pH were studied. Beer's law is obeyed in the range 2–30 ppm.The method is applied to the determination of emodin in roots, stems, and leaves of Rumex cyprius plant.