A Rapid Method for Extraction of High Quality DNA from Single Grape Seeds for PCR Use

Journal Title, Volume, Page:

Bethlehem Journal, vol. 20, 01/2001

Year of Publication:

2001

Authors:

Yaish Sami

Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine

Current Affiliation:

Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine

[email protected]

Preferred Abstract (Original):

A rapid and efficient method for DNA extraction from grape seed is described, The grinding of dry seed tissue with the aid of powdered glass produces very fine tissue fragments, this eases the treatment of the tissue with various buffers and makes them more effective in extracting the contents of the cells including DNA. The purified DNA is of high quality to the' extent that only a few samples out of hundreds prepared by this method failed to amplify in PCR.

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A Rapid Method for Extraction of High Quality DNA from Single Grape Seeds for PCR Use