Ion-Pair Spectrophotometric Determination of Dibucaine

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Year of Publication: 
Raqi Mohammad Hasan Shubietah
Current Affiliation: 
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Preferred Abstract (Original): 
A simple and sensitive spectrophotometric determination of dibucaine has been established. It is based on the formation of colored ion-pair complexes between dibucaine and each of Orange II, Orange G, Bromothymol blue and Bromocresol green. The extract with each of the previous dyes in chloroform exhibited a specific wavelength of maximum absorbance, and all these wavelengths lied in the range 416 to 500 nm. The linear range extended from 1.5 to 60 ppm. The optimum conditions were selected after studying many variables such as pH, shaking time, temperature and dye concentrations. The nmethod was also selective for the analyte and the drug excipients did not interfere. Key words: Dibucaine, Ion-pair spectrometry