Partial Characterization of a New Ampelovirus Associated With Grapevine Leafroll Disease

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Journal Title, Volume, Page: 
JOURNAL OF PLANT PATHOLOGY, 01/2004; 86(2):123-133
Year of Publication: 
2004
Authors: 
Raed Alkowni
Department of Plant Pathology, University of California, One Shields Avenue, Davis, CA 95616, USA
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
A. Rowhani
Department of Plant Pathology, University of California, One Shields Avenue, Davis, CA 95616, USA
S. Daubert
Department of Plant Pathology, University of California, One Shields Avenue, Davis, CA 95616, USA
D. Golino
Department of Plant Pathology, University of California, One Shields Avenue, Davis, CA 95616, USA
Preferred Abstract (Original): 

A virus associated with leafroll symptoms in Vitis vinifera was selected for further analysis because it was undetectable in all reverse-transcriptase polymerase chain reaction (RT-PCR) tests available for Grapevine leafroll associated viruses (GLRaVs). Extracts of infect-ed plant material were also negative in all enzyme-linked immunosorbent assays available for GLRaVs. Nonethe-less, the unknown virus caused typical grapevine leafroll symptoms in the indicator host, V. vinifera cv Cabernet Franc. High molecular weight double-stranded RNA (dsRNA) purified from an infected vine appeared typi-cal of the dsRNA recovered from plants infected by viruses in family Closteroviridae. RT-PCR was performed using this purified dsRNA with degenerate oligonu-cleotide primers designed to amplify a fragment from the heat shock protein 70 homologue (HSP70) from any closterovirus. Sequence information from the resulting RT-PCR products allowed the determination of a full length HSP70 sequence from the unknown virus for comparison with those of other GLRaVs. A specific RT-PCR assay based on primers developed from this se-quence was negative for all GLRaVs tested, other than the unknown virus. The name Grapevine leafroll associ-ated virus 9 (GLRaV-9) is tentatively proposed for the novel pathogen.

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