Molecular Analysis of a California Strain of Rupestris Stem Pitting-Associated Virus Isolated From Declining Syrah Grapevines

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Journal Title, Volume, Page: 
Archives of Virology September 2006, Volume 151, Issue 9, pp 1889-1894
Year of Publication: 
2006
Authors: 
M. F. Lima
Department of Plant Pathology, University of California, Davis, California, U.S.A
J. K. Uyemoto
USDA-ARS, University of California, Davis, California, U.S.A
Raed Alkowni
Department of Plant Pathology, University of California, Davis, California, U.S.A
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
D. Golino
Department of Plant Pathology, University of California, Davis, California, U.S.A
F. Osman
Department of Plant Pathology, University of California, Davis, California, U.S.A
Preferred Abstract (Original): 

The sequence of the genome of a Rupestris stem pitting-associated virus (RSPaV) isolated from a declining Syrah grapevine in California, designated the Syrah strain (RSPaV-SY) was determined. The genome of this strain had an overall nucleotide identity of 77% in comparison with RSPaV sequences in GenBank; the coat protein was the most conserved gene among RSPaV sequences and the replicase was the least conserved gene. Phylogenetic analysis of partial coat protein and replicase gene sequences showed RSPaV-SY clustered independently from the majority of RSPaV isolates.

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