Comparative Analyses of the Coat Protein of Newly Discovered Ampelovirus Using Computer-Assisted Phylogenetic Reconstruction and Analysis: (An Approach for Classification and Characterization)

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Journal Title, Volume, Page: 
Hebron University Research Journal. Vol.(2), No.(2), pp.(16 –26), 2006
Year of Publication: 
2006
Authors: 
Raed Alkowni
Arab American University, Jenin - Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Preferred Abstract (Original): 

The coat protein of the newly found closteroviruses Grapevine leafroll associated vir r rus 9 (GLRaV-9) was used for comparative analysis with other members of the family Closteroviridae. The amino acid alignments using ClustalW demonstrated a significant homology between the GLRaV-9 sequence and the Grapevine leafroll associated virus 5 (86%) and Pineapple mealybug wiltrassociated virusr1 (56%). GLRaV-9 and GLRaV- 5 were proved to be serologically distinct by using GCG sequence analysis software package that indicate the significant variation (26%) within their polypeptide sequences at the N-terminus of their coat protein. The phylogenetic analysis, grouped GLRaV-9 with mealybug transmitted viruses (GLRaV-1, -3, -5, and PMWaV-1, -2), suggesting the mode of the transmission of this newly found closterovirus. *

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