Isolation and Characterization of Shiga Toxigenic Escherichia Coli Strains From Northern Palestine

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Journal Title, Volume, Page: 
J. Medical Microbiology. 2002; 51: 1-4
Year of Publication: 
2002
Authors: 
Adwan K.
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Abu-Hasan N.
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Essawi T.
Department of Biological Sciences, Bir Zeit University, Palestine
Bdir M.
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
Preferred Abstract (Original): 

Shiga toxigenic Escherichia coli (STEC) isolates from symptomatic and asymptomatic patients in northern Palestine in 1999 were screened for serotype O157 and characterised for virulence genes by multiplex PCR assay. Of the 176 STEC isolates, 124 (70.5%) were of serotype O157. All these isolates carried the gene for Shiga toxin type  (stx 1 ) and 112 (90.3%) carried stx 2  . The intimin encoding gene locus eae was detected in 16 isolates (12.9%) and the enterohaemolysin encoding gene, hlyA,in18 (14.5%). Statistical analysis showed a significant association between the presence of eaeA and hlyA, either alone or combined with stx 2 and stx1 genes in O157 isolates from symptomatic infection. ERIC-PCR analysis of DNA from 80 serotype O157 isolates revealed three major clonal populations.

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