Phytochemical Screening and In-vitro Evaluation of Antioxidant and Antimicrobial Activities of the Entire Khella Plant (Ammi visnaga.L.)

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Journal Title, Volume, Page: 
International Journal of Pharmacognosy and Phytochemical Research, 7 (1)
Year of Publication: 
2015
Authors: 
Jaradat Nidal Amin
Current Affiliation: 
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine
Al-Masri Motasem
Speih Reem Ibrahem
Johari Mona Ass’ad
Awad May Ayed
Abuhasan Murad
Preferred Abstract (Original): 

Objectives: In the developing countries, a large number of people depend on the traditional folk medicine as primary substances in their healthcare systems especially rural areas healers and patients from centuries. An estimated 35,000 to 70,000 plant species are used for medicinal and therapeutic purposes in the world. The objectives of this study were to screen the phytochemical constituents and antibacterial activities also to evaluate antioxidant property of the Ammivisnaga(L.) Lam. entire plant. 

Methods: The Ammi visnaga plant was phytochemically screened (acetone, methanolic and aqueous) for the presence of phytogenic chemicals and, their effect on 2,2-Diphenyl-1-picryl-hydrazyl radical (DPPH) was used to determine their free radical scavenging activity. Broth microdilution method was applied to detect antibacterial activity and determine minimal inhibitory concentration (MIC) of aqueous and organic extracts of   Ammivisnaga (L.).  The antimicrobial activity was examined against 3 reference strains namely:  Staphylococcus aureus (ATCC 25923),Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853).  Moreover, MIC against  Acinetobacter baumannii clinical strain was included. 

Results: The results showed that Ammi visnaga methanolic extract contains a mixture of phytochemical classes as proteins, tannins, flavonoids, glycosides and steroids and revealed that this plant has high antioxidant activity (IC50=6 µg/ml). While aqueous extract exhibited antimicrobial activity against S. aureus (MIC= 25 mg/ml),  E. coli (25 mg/ml), P. aeruginosa (25 mg/ml) and  A. baumannii (50 mg/ml).  Organic extract ofAmmi visnaga (L.) showed stronger inhibition of bacterial growth, where MIC values for S. aureus, E. coli, P. aeruginosaand A. baumannii were 0.35, 2.78, 5.56 and 5.56 mg/ml, respectively. 

Conclusions: The results of the present study indicated that the Ammivisnaga (L.), especially organic extract, exhibited strong antibacterial activity against Gram-positive and Gram-negative bacterial isolates and has high antioxidant activity and therefore it can provide natural source of antibacterial drugs and antioxidants and can be useful in preventing various diseases including cancer.

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