High Performance Liquid Chromatographic Method for Quantitative Determination of Emodin in Rumex Cyprius Marb, Spectrophotometric Studies

Nidal Zatar's picture
Journal Title, Volume, Page: 
International Journal of Sciences: Basic and Applied Research (IJSBAR)1-2014
Year of Publication: 
2014
Authors: 
Ahmad Ibrahim Ahmad Husein
Mohammed S. Ali-Shtayeh
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine
waheed J. Jondi
Current Affiliation: 
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
Nidal A. Zatar
Current Affiliation: 
Department of Chemistry, Faculty of Science, An-Najah University, Nablus, Palestine
Mohammed A. Al-Nuri
Current Affiliation: 
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine
Preferred Abstract (Original): 
A quantitative method is used for determination of emodin in Rumex cyprius Marb., using high performance liquid chromatography. The plant parts: roots, stems, and leaves, were soaked in 95% methanol solution for 48 hours. Plant parts and standard emodin were eluted from C18 column with a mobile phase consisting of methanol: water (80%:20%volume/volume) at a flow rate of (2.0 milliliter / minute). The effluent was monitored with ultraviolet detector set at 258 nm. Standard curve for emodin was linear in the range of concentration 1.35- 45.0 ppm .The method was applied for determination of emodin in roots, stems and leaves of R. cyprius Marb. The results showed that the concentration of emodin in roots, stems, and leaves were 0.16%, 0.04%, and 0.30% respectively in dried plant sample. On the other hand the results obtained showed that emodin exhibits two absorption maxima at 410nm and at 510nm. The absorption peak at 410nm was found to decrease gradually by increasing pH, while the absorption peak at 510nm was found to increase gradually by increasing the pH.
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HPLC emodin Rumex BERC International Journal of Sciences Basic and Applied Research (IJSBAR) (2014) Volume 18, No 2, pp 217-222.pdf201.12 KB