An improved method for the isolation of Pythium from soil has been devised. Using this technique 45 species of Pythium have been isolated from the Reading area. Up to 8600 propagules g d.w.−1 of Pythium have been recorded and population levels exceeding 2000 propagules g d.w.−1 were frequent. Community structure of the component species populations has been assessed by Braun-Blanquet analysis and Sørensen coefficients. The former revealed species associations which could be related to taxonomic diversity within each association. The qualitative assessments from both methods and the quantitative assessment using the Sørensen coefficient indicate that locality and land management influence community structure. Soil pH is regarded as being the most important of the physico-chemical soil characteristics tested.