Comparison of qualitative, quantitative analysis and antioxidant potential between wild and cultivated Borago officinalis leaves from Palestine

Journal Title, Volume, Page:

Pakistan Journal of Pharmaceutical Sciences, 31(3)

Year of Publication:

2018

Authors:

Hassan Abu-Qaoud

Department of Plant Production and Protection, Faculty of Agriculture and Veterinary Medicine

Nuha Shawarb

Department of Chemistry, Faculty of Science, An-Najah National University Nablus, State of Palestine

Fatima Hussen

Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University Nablus, State of Palestine

Nidal Jaradat

Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University Nablus, State of Palestine

Munqez Shtaya

Department of Plant Production and Protection, Faculty of Agriculture and Veterinary Medicine

Preferred Abstract (Original):

Borago officinalis plant is an important plant of high medicinal and nutritional values. This study designed to

evaluate antioxidant activity, screen the existence of phytogenic chemical compounds and to determine the total

flavonoid and phenol contents of wild and cultivated Borago officinalis. Total flavonoid contents of the wild and

cultivated Borago officinalis were determined by using rutin reference standard method and total phenols determined by

using Folin Ciocalteu’s method while antioxidant activity evaluated by using 2, 2-diphenyl-1-picryl-hydrazyl-hydrate

assay. Phytochemical analyses indicated the presence of carbohydrate, phenols, flavonoids, phytosteroids tannins and

volatile oil. The total flavonoid content of the methanolic extract from the wild borage plant was 22.4mg RU/g this value

was reduced to 13.1mg RU/g for the cultivated methanolic extract as well as the total phenols contents was dropped from

5.21mg GA/g to 2.37mg GA/g methanolic extracts. Total tannins content of the wild growing borage plant was 13.7mg

GA/g methanolic extract. This value was higher in the cultivated borage with 21.33mg GA/g methanolic extract. The

wild leaves extract had IC50 =6.3μg/mL for wild leaves extract was closer to IC50 value of Trolox (standard reference

with high antioxidant activity), while the cultivated leaves extract had higher IC50= 8.7μg/ml which mean lower

antioxidant activity than the wild growing one. The data of this study showed that the extracts of Borago officinalis

possess antioxidant and free radical scavenging activities. Variation was clear between wild and cultivated species, these

findings propose that such plant extract could have a wide range of applications in both food and pharmaceutical

industries. Therefore, more research is necessary to investigate different cultural practices on the efficiency of borage

plant.

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An-Najah Staff