Plumbagin a Naturally Occurring Naphthoquinone: Its Isolation, Spectrophotometric ‎Determination in Roots, Stems, and Leaves in Plumbago Europea L

Journal Title, Volume, Page:

Spectroscopy Letters, Volume 27, Issue 4, pages 409 - 416

Year of Publication:

1994

Link:

http://blogs.najah.edu/staff/nidalzatar/article/Plumbagin-a-Naturally-Occurring-...

Authors:

Mohammed A. Al-Nuri

Chemistry Department, An-Najah National University, Nablus, Palestine

Current Affiliation:

Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, Palestine

[email protected]

Maher A. Abu-Eid

Chemistry Department, An-Najah National University, Nablus, Palestine

Nidal A. Zatar

Chemistry Department, An-Najah National University, Nablus, Palestine

Waheed J. Al-Jondi

Chemistry Department, An-Najah National University, Nablus, Palestine

Mohammed A. Hannoun

Chemistry Department, An-Najah National University, Nablus, Palestine

Mohammed S. Ali-Shtayeh

Biology Department, An-Najah National University, Nablus, Palestine

Preferred Abstract (Original):

A new method for isolation and spectrophotometric determination of plumbagin is presented. Plumbagin was isolated by thin layer chromatography (TLC) and column chromatography (CC) techniques, as an orange tinged yellow long crystalline substances. Plumbagin exhibits two absrop-tion maxima at 410 and 510 nm. Stability of the color, pKa value, and the effect of pH were studied. Beer's law is obeyed over the range 0.9-45 ppm. The method is applied to the determination of plumbagin in roots, stems, and leaves of Plumbago europaea L. plant.

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Plumbagin a Naturally Occurring Naphthoquinone: Its Isolation, Spectrophotometric ‎Determination in Roots, Stems, and Leaves in Plumbago Europea L