The Antibacterial activity of parsley (Petroselinum crispum) and Smooth Sumac ( Rhus glabra) leaves aqueous extract were examined using agar disc diffusion methods against six bacteria ( Bacillus subtilis , Staphylococcus aureas ,Escherichia coli . proteus mirabilis , Klebssialla pneumonia , Pseudomonas aeruginsa ) . The extract of both plants had inhibitory effect at various concentration ( 4%,5%,7%) for sumac and ( 1.5%, 2%) for Parsley against both Gram(+)and Gram(-) . Based on these results of this study both plants could be considered as potential antibacterial agents which confirms their use in folk Medicine .