In vitro REGENERATION OF TOMATO (Lycopersicon esculentum Mill)

Journal Title, Volume, Page:

Plant Cell Biotechnology and Molecular Biology 16(3&4):181-190

Year of Publication:

2015

Authors:

FEDAA JAMOUS

HASSAN ABU-QAOUD

Preferred Abstract (Original):

A protocol was developed for shoot multiplication and regeneration in two tomato (Lycopersicon

esculentum) cultivars. Shoots tips of about 2 cm length from in vitro establishment seedlings were

used in the multiplication experiments. The explants were transferred into Murashige and Skoog

media (MS medium) with (2.2, 4.4 μM) Benzyl adenine (BA), (9.2, 18.4 μM) Kinetin combined with

0.0, 2.7 μM Naphthalene acetic acid (NAA). Higher shoot number (8.4) was obtained with MS

medium supplied with 18.6 μM Kinetin. For regeneration experiments, hypocotyl, cotyledon, stem,

and leaf explants of both cultivars were used. Explants were cultured on MS media supplied with

different levels of Naphthalene acetic acid (NAA) and Benzyl adenine (BA), Kinetin (Kin) and N-

1,2,3-Thiadiazol-5-yl-N’-phenylurea(TDZ) Direct regeneration from the different expants was

obtained on MS basal medium supplemented with TDZ at (0, 1, 2 and 4 μM) and NAA at (0, 2.7 and

5.4 μM), or BA at (0.0 or 2.2 μM) and Kinetin at (0.0, 2.3 μM). The highest shoot % (62.25) was

obtained when Kinetin and BA were used at (2.3 and 2.2 μM) respectively. However, when NAA

and TDZ were combined, 39.9 and 46.91% shoot regeneration was achieved with 2.7 and 4 μM,

respectively for both Baladi and 593 cultivars. Very low shoot regeneration was observed with all

NAA levels combined with 1 and 2 μM TDZ.

An-Najah Staff