Influence of Nitrogen form and NH4 +-N/:NO3 −-N Ratios on Adventitious Shoot Formation from Pear (Pyrus communis) Leaf Explants in Vitro

Journal Title, Volume, Page:

Plant Cell, Tissue and Organ Culture, Volume 27, Issue 3, pp 315-319

Year of Publication:

1991

Link:

http://link.springer.com/article/10.1007%2FBF00157597

Authors:

Hassan Abu-Qaoud

Department of Plant Production, Faculty of Agriculture, University of Jordan, Amman, Jordan

Current Affiliation:

Department of Plant Production and Protection,Faculty of Agriculture and Veterinary Medicine, An Najah National University, Nablus, Palestine

[email protected]

R. M. Skirvin

Department of Horticulture, University of Illinois, 258 Plant and Animal Biotechnology Laboratory, 1201 Gregory Dr., 61801, Urbana, IL, USA

F. E. Below

Department of Agronomy, University of Illinois, 322 Plant and Animal Biotechnology Laboratory, 1201 Gregory Dr., 61801, Urbana, IL, USA

Preferred Abstract (Original):

The effect of various basal salts media, containing different nitrogen levels on in vitro adventitious shoot regeneration from leaf explants of ‘Louise Bonne Panachee’ and ‘Seckel’ pear (Pyrus communis L.) were investigated. Among the different basal salt formulae tested, Nitsch (1969) gave significantly better regeneration in most of the experiments. Shoot regeneration was altered with different NH4 +-N/NO3 −-N ratios. The best regeneration was obtained when NH4 +:NO3 − was either 1:2 or 1:3 regardless of overall N concentration. In addition, these data show that NH4 + was essential for adventitious shoot regeneration from pear leaf explants on White's (1943) medium.

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Influence of Nitrogen form and NH4 +-N/:NO3 −-N Ratios on Adventitious Shoot Formation from Pear (Pyrus communis) Leaf Explants in Vitro