Home

Spectrophotometric Determination

MSShtayeh's picture

Emodin, A Naturally Occuring Anthraquinone: Its Isolation and Spectrophotometric Determination in Rumex Cyprius Plant

Tue, 2014-02-18 10:19 — Mohammed Saleem Ali-Shtayeh
Journal Title, Volume, Page: 
Spectroscopy Letters: An International Journal for Rapid Communication Volume 29, Issue 8,pages 1539-1543, DOI: 10.1080/00387019608007144, 1996
Year of Publication: 
1996
Link: 
http://www.tandfonline.com/doi/abs/10.1080/00387019608007144#preview
Authors: 
Mohammed A. Al-Nuri
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus. Palestine
Nidal A. Za'tar
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus. Palestine
Maher A. Abu-Eid
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus. Palestine
Mohammad A. Hannoun
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus. Palestine
Waheed J. Al-Jondi
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus. Palestine
Ahmad I. Hussein
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus. Palestine
Mohammad S. Ali-Shtayeh
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus. Palestine
Current Affiliation: 
Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus. Palestine
[email protected]
Preferred Abstract (Original): 

A new method for isolation and specrophotometric determination of emodin is presented. Emodin was isolated by thin layer chromatography (tlc) and column chromatography (cc) techniques, as an orange long crystalline substance. Emodin exhibits two absorption maxima, at 420 and 520 nm. Stability of the color and the effect of pH were studied. Beer's law is obeyed in the range 2–30 ppm. The method is applied to the determination of emodin in roots, stems, and leaves of Rumex cyprius plant.

Syndicate content