Mode of action of the antimicrobial compound 5-Bromo-5-nitro-1,3-dioxane (Bronidox)

Journal Title, Volume, Page:

Folia Microbiologica February 1986, Volume 31, Issue 1, pp 19-31

Year of Publication:

1986

Link:

http://link.springer.com/article/10.1007/BF02928676

Authors:

M. Ghannoum

Department of Botany and Microbiology, Kuwait University, Faculty of Science, Kuwait

M. Thomson

Department of Biochemistry, Kuwait University, Faculty of Science, Kuwait

W. Bowman

Department of Chemistry, University of Technology, Loughborough, UK

S. Al-Khalil

Department of Chemistry, University of Technology, Loughborough, UK

Current Affiliation:

Department of Medical Laboratory Sciences, Faculty of Science, An-Najah National University, Palestine

[email protected]

Preferred Abstract (Original):

The mode of action of the antimicrobial agent, 5-bromo-5-nitro-l,3-dioxane (bronidox), was studied in detail for gram-positive and gram-negative bacteria, yeast and fungi. The studies included MIC testing, thiol inhibition of activity, intracellular leakage, oxygen consumption, incorporation of³H-uridine, scanning electron microscopy, inhibition of enzyme activity (papain) andin vitro oxidation of thiols to disulfides. It appears that the primary mode of action of bronidox is the same as, or similar to, that of bronopol,i.e. the oxidation of essential protein thiol causing inhibition of enzyme activity and subsequent inhibition of microbial growth.

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Mode of action of the antimicrobial compound 5-Bromo-5-nitro-1,3-dioxane (Bronidox)