The Use Of SDS Polyacrylamide Gel Electrophoresis Of Periplasmic Proteins To Subtype Pseudomonas Aeruginosa Pyocin Type 10/B Clinical Isolates

Journal Title, Volume, Page:

Infection. 1999 Jan-Feb;27(1):39-41

Year of Publication:

1999

Link:

http://www.ncbi.nlm.nih.gov/pubmed/10027106

Authors:

K. Adwan

Dept. of Biological Sciences, An-Najah National University, P.O. Box 7, Nablus, Palestine

Current Affiliation:

Department of Biology, Faculty of Science, An-Najah National University, Nablus. Palestine

[email protected]

Preferred Abstract (Original):

The periplasmic protein banding patterns (PPBPs) of thirteen strains of Pseudomonas aeruginosa pyocin type 10/b implicated in two nosocomial outbreaks in the neonatal unit of Rafeidia Hospital, Nablus, Palestine, were examined. In addition, five strains from sporadic cases from the same unit occurring in 1996 and 1997 were also studied. Despite different sources of the strains, PPBPs generated by PAGE suggested a clonal nature of the strains obtained during each of the two outbreaks. Although they had very similar PPBPs, the two outbreak clones were not identical. In contrast, sporadic strains of P. aeruginosa pyocin type 10 appeared to be much more heterogeneous than those of the two outbreaks. PPBP analysis appeared to be a useful tool that may be of value for epidemiological purposes.

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The Use Of SDS Polyacrylamide Gel Electrophoresis Of Periplasmic Proteins To Subtype Pseudomonas Aeruginosa Pyocin Type 10/B Clinical Isolates